Version 5.4
Homo sapiens Gene: GCLC
Summary
InnateDB Gene IDBG-91041.5
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol GCLC
Gene Name glutamate-cysteine ligase, catalytic subunit
Synonyms GCL; GCS; GLCL; GLCLC
Species Homo sapiens
Ensembl Gene ENSG00000001084
Encoded Proteins
IDBP-91043
glutamate-cysteine ligase, catalytic subunit
IDBP-473981
glutamate-cysteine ligase, catalytic subunit
IDBP-477365
glutamate-cysteine ligase, catalytic subunit
IDBP-480979
glutamate-cysteine ligase, catalytic subunit
IDBP-483884
glutamate-cysteine ligase, catalytic subunit
IDBP-479692
glutamate-cysteine ligase, catalytic subunit
IDBP-482600
glutamate-cysteine ligase, catalytic subunit
IDBP-483963
glutamate-cysteine ligase, catalytic subunit
IDBP-807259
glutamate-cysteine ligase, catalytic subunit
Protein Structure
Useful resources Stemformatics EHFPI ImmGen
Entrez Gene
Summary Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
Gene Information
Type Protein coding
Genomic Location Chromosome 6:53497341-53616970
Strand Reverse strand
Band p12.1
Transcripts
ENST00000229416 ENSP00000229416
ENST00000509541
ENST00000515580
ENST00000510837
ENST00000504353
ENST00000514373 ENSP00000426578
ENST00000504525 ENSP00000421228
ENST00000514004 ENSP00000421908
ENST00000505294 ENSP00000421629
ENST00000513939 ENSP00000424211
ENST00000514933 ENSP00000423615
ENST00000505197 ENSP00000427403
ENST00000616923 ENSP00000482756
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 15 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.
Experimentally validated
Total 15 [view]
Protein-Protein 7 [view]
Protein-DNA 7 [view]
Protein-RNA 1 [view]
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 1 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0000287 magnesium ion binding
GO:0004357 glutamate-cysteine ligase activity
GO:0005524 ATP binding
GO:0016595 glutamate binding
GO:0043531 ADP binding
GO:0046982 protein heterodimerization activity
GO:0050662 coenzyme binding
Biological Process
GO:0000096 sulfur amino acid metabolic process
GO:0006534 cysteine metabolic process
GO:0006536 glutamate metabolic process
GO:0006749 glutathione metabolic process
GO:0006750 glutathione biosynthetic process
GO:0006805 xenobiotic metabolic process
GO:0006979 response to oxidative stress
GO:0008637 apoptotic mitochondrial changes
GO:0009408 response to heat
GO:0009410 response to xenobiotic stimulus
GO:0009725 response to hormone
GO:0019852 L-ascorbic acid metabolic process
GO:0031397 negative regulation of protein ubiquitination
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0034641 cellular nitrogen compound metabolic process
GO:0043066 negative regulation of apoptotic process
GO:0043524 negative regulation of neuron apoptotic process
GO:0044281 small molecule metabolic process
GO:0045454 cell redox homeostasis
GO:0045892 negative regulation of transcription, DNA-templated
GO:0046685 response to arsenic-containing substance
GO:0050880 regulation of blood vessel size
GO:0051409 response to nitrosative stress
GO:0051900 regulation of mitochondrial depolarization
GO:1901687 glutathione derivative biosynthetic process
GO:2001237 negative regulation of extrinsic apoptotic signaling pathway
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0017109 glutamate-cysteine ligase complex
Orthologs
Species
Mus musculus
Bos taurus
Gene ID
Gene Order
ENSMUSG00000032350
View Gene Order
ENSBTAG00000015571
Not yet available
Pathways
NETPATH
REACTOME
REACT_115639
Sulfur amino acid metabolism pathway
REACT_6960
Glutathione synthesis and recycling pathway
REACT_6926
Glutathione conjugation pathway
REACT_13
Metabolism of amino acids and derivatives pathway
REACT_6959
Phase II conjugation pathway
REACT_111217
Metabolism pathway
REACT_13433
Biological oxidations pathway
KEGG
hsa00480
Glutathione metabolism pathway
INOH
INOH website
Glutamate Glutamine metabolism pathway
PID NCI
Cross-References
SwissProt
TrEMBL
UniProt Splice Variant
Entrez Gene
UniGene Hs.596571 Hs.623046 Hs.654465
RefSeq NM_001197115 NM_001498
HUGO
OMIM
CCDS CCDS4952 CCDS75471
HPRD 06032
IMGT
EMBL
GenPept
RNA Seq Atlas

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca