Mus musculus Gene: Sqstm1
Summary
InnateDB Gene IDBG-168358.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol Sqstm1
Gene Name sequestosome 1
Synonyms A170; OSF-6; Osi; p62; STAP; STONE14;
Species Mus musculus
Ensembl Gene ENSMUSG00000015837
Encoded Proteins
sequestosome 1
sequestosome 1
sequestosome 1
sequestosome 1
Protein Structure
Useful resources Stemformatics EHFPI ImmGen
InnateDB Annotation
Summary
Sqstm1 is required for Tlr4-mediated autophagy. Tlr4-driven induction of Sqstm1 plays an essential role in the formation and the autophagy degradation of aggresome-like induced structures, which might be critical for regulating host defense.
Sqstm1 and Calcoco2 are ubiquitin-autophagy receptors that are required for the recognition of extracelluar bacterial DNA by the Tmem173 (STING)-dependent cytosolic pathway, marking bacteria with ubiquitin, and delivery of bacilli to autophagosomes.
Phosphorylation of Sqstm1 (p62) activates the Keap1-Nrf2 pathway during selective autophagy.
Sqstm1 captures Tnfaip3, an NFkB inhibitor, and sequesters it in the autophagosome. This allows macrophages to release chemokines that recruit neutrophils and boost antifungal immunity.
InnateDB Annotation from Orthologs
Summary
[Homo sapiens] SQSTM1 (p62) targets invading bacteria to the autophagy pathway and its expression is required for efficient autophagy of bacteria, as well as restriction of their intracellular replication.
[Homo sapiens] SQSTM1 is a key intracellular target of innate defence regulator-1 (IDR-1), a synthetic peptide.
[Homo sapiens] SQSTM1 has bactericidal properties where it brings cytosolic proteins to autolysosomes where they are processed from innocuous precursors into neo-antimicrobial peptides.
[Homo sapiens] SQSTM1 and HDAC6 are important determinants of aggregated localization of MyD88 and MyD88 activation initiates a polyubiquitinated protein accumulating pathway that modulates MyD88-dependent signal transduction.
[Homo sapiens] SQSTM1 is required for TLR4-mediated autophagy. TLR4-driven induction of SQSTM1 plays an essential role in the formation and the autophagy degradation of aggresome-like induced structures, which might be critical for regulating host defence.
[Homo sapiens] SQSTM1 and CALCOCO2 are ubiquitin-autophagy receptors that are required for the recognition of extracelluar bacterial DNA by the TMEM173 (STING)-dependent cytosolic pathway, marking bacteria with ubiquitin, and delivery of bacilli to autophagosomes. (Demonstrated in mouse)
Entrez Gene
Summary This gene does not have any Entrez summary - the following is the summary from its human ortholog ENSG00000161011:
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Gene Information
Type Protein coding
Genomic Location Chromosome 11:50199366-50210827
Strand Reverse strand
Band B1.3
Transcripts
ENSMUST00000102774 ENSMUSP00000099835
ENSMUST00000015981 ENSMUSP00000015981
ENSMUST00000136936 ENSMUSP00000120442
ENSMUST00000143379 ENSMUSP00000118662
ENSMUST00000131214
ENSMUST00000147846
ENSMUST00000154805
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 98 experimentally validated interaction(s) in this database.
They are also associated with 172 interaction(s) predicted by orthology.
Experimentally validated
Total 98 [view]
Protein-Protein 98 [view]
Protein-DNA 0
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 172 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003712 transcription cofactor activity
GO:0005080 protein kinase C binding
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0019901 protein kinase binding
GO:0042169 SH2 domain binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0043130 ubiquitin binding
GO:0070530 K63-linked polyubiquitin binding
Biological Process
GO:0001934 positive regulation of protein phosphorylation
GO:0002376 immune system process
GO:0006914 autophagy
GO:0006915 apoptotic process
GO:0016236 macroautophagy
GO:0016239 positive regulation of macroautophagy
GO:0030154 cell differentiation
GO:0043122 regulation of I-kappaB kinase/NF-kappaB signaling
GO:0045087 innate immune response (InnateDB)
GO:0051291 protein heterooligomerization
GO:2001141 regulation of RNA biosynthetic process
Cellular Component
GO:0000407 pre-autophagosomal structure
GO:0000932 cytoplasmic mRNA processing body
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005764 lysosome
GO:0005770 late endosome
GO:0005776 autophagic vacuole
GO:0005783 endoplasmic reticulum
GO:0016234 inclusion body
GO:0016235 aggresome
GO:0016605 PML body
GO:0031410 cytoplasmic vesicle
GO:0070062 extracellular vesicular exosome
Orthologs
Species
Homo sapiens
Bos taurus
Gene ID
Gene Order
Method
Confidence
Comments
SSD Ortholog
Ortholog supports species divergence
Not yet available
SSD Ortholog
Ortholog supports species divergence
Pathways
NETPATH
REACTOME
Cytokine Signaling in Immune system pathway
Immune System pathway
Cell death signalling via NRAGE, NRIF and NADE pathway
p75NTR recruits signalling complexes pathway
Signaling by Interleukins pathway
p75NTR signals via NF-kB pathway
Signalling by NGF pathway
NF-kB is activated and signals survival pathway
Interleukin-1 signaling pathway
Signal Transduction pathway
p75 NTR receptor-mediated signalling pathway
NRIF signals cell death from the nucleus pathway
KEGG
Osteoclast differentiation pathway
INOH
PID BIOCARTA
PID NCI
Pathway Predictions based on Human Orthology Data
NETPATH
IL1 pathway
RANKL pathway
REACTOME
Signalling by NGF pathway
p75NTR recruits signalling complexes pathway
Cytokine Signaling in Immune system pathway
NF-kB is activated and signals survival pathway
p75 NTR receptor-mediated signalling pathway
p75NTR signals via NF-kB pathway
Signal Transduction pathway
Immune System pathway
NRIF signals cell death from the nucleus pathway
Signaling by Interleukins pathway
Interleukin-1 signaling pathway
Cell death signalling via NRAGE, NRIF and NADE pathway
KEGG
Osteoclast differentiation pathway
INOH
PID BIOCARTA
PID NCI
IL1-mediated signaling events
TNF receptor signaling pathway
Neurotrophic factor-mediated Trk receptor signaling
p75(NTR)-mediated signaling
Cross-References
SwissProt
TrEMBL
UniProt Splice Variant
Entrez Gene
UniGene Mm.400065 Mm.40828 Mm.473573
RefSeq NM_001290769 NM_011018 XM_006532450
OMIM
CCDS CCDS24629 CCDS70176
HPRD
IMGT
MGI ID
MGI Symbol
EMBL
GenPept
RNA Seq Atlas