Homo sapiens Gene: NOS2
Summary
InnateDB Gene IDBG-36000.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol NOS2
Gene Name nitric oxide synthase 2, inducible
Synonyms HEP-NOS; INOS; NOS; NOS2A;
Species Homo sapiens
Ensembl Gene ENSG00000007171
Encoded Proteins
nitric oxide synthase 2, inducible
nitric oxide synthase 2, inducible
Protein Structure
Useful resources Stemformatics EHFPI ImmGen
InnateDB Annotation
Summary
NOS2 is a nitric oxide synthase (iNOS) protein and combined treatment with 1,25-dihydroxyvitamin D3 (1,25-D3) and IFN-gamma has been shown to synergistically enhance NO synthesis and NOS2 expression induced by Mycobacterium tuberculosis (MTB) or by its purified protein derivatives in human monocyte-derived macrophages.
NOS2 is an inducible nitric oxide (NO) synthase present in innate immune cells that produces NO in response to certain infections or upon stimulation with cytokines such as IFN-gamma and TNF. For optimal induction of NOS2 during Chlamydophila pneumoniae infection, the concerted action of the MyD88-dependent transcription factors NF-kappaB and AP-1 and of the MyD88-independent transcription factors phosphorylated STAT1 and IRF1 is required.
NOS2 (iNOS) and CALM1 (CaM) coordinately function to form a stable complex that is part of a rapid host response that functions within the first 30 min following bacterial infection to up-regulate the innate immune system involving macrophage activation.
Nitric oxide production by NOS2 promotes Listeria monocytogenes dissemination in the host. (Demonstrated in mice)
InnateDB Annotation from Orthologs
Summary
[Mus musculus] Nos2 (iNOS) and Calm1 (CaM) coordinately function to form a stable complex that is part of a rapid host response that functions within the first 30 min following bacterial infection to upregulate the innate immune system involving macrophage activation.
[Mus musculus] Nitric oxide production by Nos2 promotes Listeria monocytogenes dissemination in the host.
[Mus musculus] Inorganic polyphosphate suppresses Nos2 expression and nitric oxide release but not TNF release in LPS-activated macrophages
Entrez Gene
Summary Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Gene Information
Type Protein coding
Genomic Location Chromosome 17:27756766-27800499
Strand Reverse strand
Band q11.2
Transcripts
ENST00000313735 ENSP00000327251
ENST00000621962 ENSP00000482291
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 182 experimentally validated interaction(s) in this database.
They are also associated with 37 interaction(s) predicted by orthology.
Experimentally validated
Total 182 [view]
Protein-Protein 163 [view]
Protein-DNA 18 [view]
Protein-RNA 1 [view]
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 37 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003958 NADPH-hemoprotein reductase activity
GO:0004517 nitric-oxide synthase activity
GO:0005102 receptor binding
GO:0005506 iron ion binding
GO:0005515 protein binding
GO:0005516 calmodulin binding
GO:0010181 FMN binding
GO:0016491 oxidoreductase activity
GO:0020037 heme binding
GO:0034617 tetrahydrobiopterin binding
GO:0034618 arginine binding
GO:0042803 protein homodimerization activity
GO:0050660 flavin adenine dinucleotide binding
GO:0050661 NADP binding
Biological Process
GO:0001666 response to hypoxia
GO:0001912 positive regulation of leukocyte mediated cytotoxicity
GO:0002227 innate immune response in mucosa
GO:0006527 arginine catabolic process
GO:0006801 superoxide metabolic process
GO:0006809 nitric oxide biosynthetic process
GO:0006954 inflammatory response
GO:0007263 nitric oxide mediated signal transduction
GO:0007596 blood coagulation
GO:0007623 circadian rhythm
GO:0009617 response to bacterium
GO:0010629 negative regulation of gene expression
GO:0018119 peptidyl-cysteine S-nitrosylation
GO:0031284 positive regulation of guanylate cyclase activity
GO:0032496 response to lipopolysaccharide
GO:0042127 regulation of cell proliferation
GO:0042177 negative regulation of protein catabolic process
GO:0042742 defense response to bacterium
GO:0043457 regulation of cellular respiration
GO:0045087 innate immune response (InnateDB)
GO:0045776 negative regulation of blood pressure
GO:0045909 positive regulation of vasodilation
GO:0050796 regulation of insulin secretion
GO:0050829 defense response to Gram-negative bacterium
GO:0051701 interaction with host
GO:0051712 positive regulation of killing of cells of other organism
GO:0055114 oxidation-reduction process
GO:0071222 cellular response to lipopolysaccharide
GO:0071346 cellular response to interferon-gamma
GO:0090382 phagosome maturation
Cellular Component
GO:0005622 intracellular
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005777 peroxisome
GO:0005829 cytosol
GO:0030863 cortical cytoskeleton
GO:0048471 perinuclear region of cytoplasm
Orthologs
Species
Mus musculus
Bos taurus
Gene ID
Gene Order
Method
Confidence
Comments
SSD Ortholog
Ortholog supports species divergence
Not yet available
SSD Ortholog
Ortholog supports species divergence
Pathways
NETPATH
REACTOME
Phagosomal maturation (early endosomal stage) pathway
Platelet homeostasis pathway
Latent infection of Homo sapiens with Mycobacterium tuberculosis pathway
Disease pathway
Hemostasis pathway
Nitric oxide stimulates guanylate cyclase pathway
KEGG
Arginine and proline metabolism pathway
Calcium signaling pathway pathway
Peroxisome pathway
Leishmaniasis pathway
Chagas disease (American trypanosomiasis) pathway
Toxoplasmosis pathway
Amoebiasis pathway
Pathways in cancer pathway
Small cell lung cancer pathway
INOH
Arginine Proline metabolism pathway
PID BIOCARTA
No2-dependent il-12 pathway in nk cells [Biocarta view]
Mechanism of gene regulation by peroxisome proliferators via ppara [Biocarta view]
PID NCI
IL12-mediated signaling events
Alpha9 beta1 integrin signaling events
ATF-2 transcription factor network
IL23-mediated signaling events
HIF-1-alpha transcription factor network
Signaling mediated by p38-alpha and p38-beta
Cross-References
SwissProt
TrEMBL Q9UM94
UniProt Splice Variant
Entrez Gene 4843
UniGene
RefSeq NM_000625
HUGO HGNC:7873
OMIM 163730
CCDS CCDS11223
HPRD
IMGT
EMBL S76479
GenPept AAD14226
RNA Seq Atlas 4843