InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Gene: NOS2

Summary

InnateDB Gene

IDBG-36000.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NOS2

Gene Name

nitric oxide synthase 2, inducible

Synonyms

HEP-NOS; INOS; NOS; NOS2A

Species

Homo sapiens

Ensembl Gene

ENSG00000007171

Encoded Proteins

IDBP-36002

nitric oxide synthase 2, inducible

IDBP-808942

nitric oxide synthase 2, inducible

Protein Structure

Useful resources

Stemformatics EHFPI ImmGen

InnateDB Annotation

Summary

PMID 20157607

NOS2 is a nitric oxide synthase (iNOS) protein and combined treatment with 1,25-dihydroxyvitamin D3 (1,25-D3) and IFN-gamma has been shown to synergistically enhance NO synthesis and NOS2 expression induced by Mycobacterium tuberculosis (MTB) or by its purified protein derivatives in human monocyte-derived macrophages.

PMID 18799752

NOS2 is an inducible nitric oxide (NO) synthase present in innate immune cells that produces NO in response to certain infections or upon stimulation with cytokines such as IFN-gamma and TNF. For optimal induction of NOS2 during Chlamydophila pneumoniae infection, the concerted action of the MyD88-dependent transcription factors NF-kappaB and AP-1 and of the MyD88-independent transcription factors phosphorylated STAT1 and IRF1 is required.

PMID 16893173

NOS2 (iNOS) and CALM1 (CaM) coordinately function to form a stable complex that is part of a rapid host response that functions within the first 30 min following bacterial infection to up-regulate the innate immune system involving macrophage activation.

PMID 22542147

Nitric oxide production by NOS2 promotes Listeria monocytogenes dissemination in the host. (Demonstrated in mice)

InnateDB Annotation from Orthologs

Summary

PMID 16893173

[Mus musculus] Nos2 (iNOS) and Calm1 (CaM) coordinately function to form a stable complex that is part of a rapid host response that functions within the first 30 min following bacterial infection to upregulate the innate immune system involving macrophage activation.

PMID 22542147

[Mus musculus] Nitric oxide production by Nos2 promotes Listeria monocytogenes dissemination in the host.

PMID 24040305

[Mus musculus] Inorganic polyphosphate suppresses Nos2 expression and nitric oxide release but not TNF release in LPS-activated macrophages

Entrez Gene

Summary

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Gene Information

Type

Protein coding

Genomic Location

Chromosome 17:27756766-27800499

Strand

Reverse strand

Band

q11.2

Transcripts

ENST00000313735	ENSP00000327251
ENST00000621962	ENSP00000482291

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 182 experimentally validated interaction(s) in this database.
They are also associated with 37 interaction(s) predicted by orthology.

Experimentally validated
Total	182 [view]
Protein-Protein	163 [view]
Protein-DNA	18 [view]
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	37 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003958	NADPH-hemoprotein reductase activity
GO:0004517	nitric-oxide synthase activity
GO:0005102	receptor binding
GO:0005506	iron ion binding
GO:0005515	protein binding
GO:0005516	calmodulin binding
GO:0010181	FMN binding
GO:0016491	oxidoreductase activity
GO:0020037	heme binding
GO:0034617	tetrahydrobiopterin binding
GO:0034618	arginine binding
GO:0042803	protein homodimerization activity
GO:0050660	flavin adenine dinucleotide binding
GO:0050661	NADP binding

Biological Process

GO:0001666	response to hypoxia
GO:0001912	positive regulation of leukocyte mediated cytotoxicity
GO:0002227	innate immune response in mucosa
GO:0006527	arginine catabolic process
GO:0006801	superoxide metabolic process
GO:0006809	nitric oxide biosynthetic process
GO:0006954	inflammatory response
GO:0007263	nitric oxide mediated signal transduction
GO:0007596	blood coagulation
GO:0007623	circadian rhythm
GO:0009617	response to bacterium
GO:0010629	negative regulation of gene expression
GO:0018119	peptidyl-cysteine S-nitrosylation
GO:0031284	positive regulation of guanylate cyclase activity
GO:0032496	response to lipopolysaccharide
GO:0042127	regulation of cell proliferation
GO:0042177	negative regulation of protein catabolic process
GO:0042742	defense response to bacterium
GO:0043457	regulation of cellular respiration
GO:0045087	innate immune response (InnateDB)
GO:0045776	negative regulation of blood pressure
GO:0045909	positive regulation of vasodilation
GO:0050796	regulation of insulin secretion
GO:0050829	defense response to Gram-negative bacterium
GO:0051701	interaction with host
GO:0051712	positive regulation of killing of cells of other organism
GO:0055114	oxidation-reduction process
GO:0071222	cellular response to lipopolysaccharide
GO:0071346	cellular response to interferon-gamma
GO:0090382	phagosome maturation

Cellular Component

GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005777	peroxisome
GO:0005829	cytosol
GO:0030863	cortical cytoskeleton
GO:0048471	perinuclear region of cytoplasm