InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RAD51B

Summary

InnateDB Protein

IDBP-10141.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RAD51B

Protein Name

RAD51 homolog B (S. cerevisiae)

Synonyms

R51H2; RAD51L1; REC2;

Species

Homo sapiens

Ensembl Protein

ENSP00000375101

InnateDB Gene

IDBG-10135 (RAD51B)

Protein Structure

UniProt Annotation

Function

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. {ECO:0000269PubMed:11751635, ECO:0000269PubMed:11751636, ECO:0000269PubMed:11842113, ECO:0000269PubMed:12441335, ECO:0000269PubMed:23108668, ECO:0000269PubMed:23149936}.

Subcellular Localization

Nucleus {ECO:0000305}.

Disease Associations

Note=A chromosomal aberration involving RAD51B is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23- 24) with HMGA1.Note=A chromosomal aberration involving RAD51B is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with HMGA2.

Tissue Specificity

Expressed in a wide range of tissues.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 25 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	25 [view]
Protein-Protein	25 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0000400	four-way junction DNA binding
GO:0003677	DNA binding
GO:0003678	DNA helicase activity
GO:0003690	double-stranded DNA binding
GO:0003697	single-stranded DNA binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008094	DNA-dependent ATPase activity

Biological Process

GO:0000724	double-strand break repair via homologous recombination
GO:0006200	ATP catabolic process
GO:0006259	DNA metabolic process
GO:0006260	DNA replication
GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0007131	reciprocal meiotic recombination
GO:0007596	blood coagulation
GO:0010971	positive regulation of G2/M transition of mitotic cell cycle

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005657	replication fork
GO:0033063	Rad51B-Rad51C-Rad51D-XRCC2 complex

Protein Structure and Domains

PDB ID

InterPro

IPR007694 DNA helicase, DnaB-like, C-terminal
IPR010995 DNA repair Rad51/transcription factor NusA, alpha-helical
IPR013632 DNA recombination and repair protein Rad51, C-terminal
IPR014774 Circadian clock protein KaiC/DNA repair protein RadA
IPR020588 DNA recombination and repair protein RecA-like, ATP-binding domain
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF03796
PF08423
PF06745

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

O15315

PhosphoSite