InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: SETDB1

Summary

InnateDB Protein

IDBP-102214.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SETDB1

Protein Name

SET domain, bifurcated 1

Synonyms

ESET; H3-K9-HMTase4; KG1T; KMT1E; TDRD21;

Species

Homo sapiens

Ensembl Protein

ENSP00000271640

InnateDB Gene

IDBG-102210 (SETDB1)

Protein Structure

UniProt Annotation

Function

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. {ECO:0000269PubMed:12869583, ECO:0000269PubMed:14536086, ECO:0000269PubMed:15327775, ECO:0000269PubMed:17952062}.

Subcellular Localization

Nucleus. Chromosome. Note=Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.

Disease Associations

Tissue Specificity

Widely expressed. High expression in testis.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 165 experimentally validated interaction(s) in this database.
They are also associated with 10 interaction(s) predicted by orthology.

Experimentally validated
Total	165 [view]
Protein-Protein	164 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	10 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0018024	histone-lysine N-methyltransferase activity

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0001833	inner cell mass cell proliferation
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0034968	histone lysine methylation
GO:0036124	histone H3-K9 trimethylation
GO:0060348	bone development

Cellular Component

GO:0000790	nuclear chromatin
GO:0005634	nucleus
GO:0005694	chromosome
GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0005886	plasma membrane

Protein Structure and Domains

PDB ID

InterPro

IPR001214 SET domain
IPR001739 Methyl-CpG DNA binding
IPR002999 Tudor domain
IPR003606 Pre-SET zinc-binding sub-group
IPR003616 Post-SET domain
IPR007728 Pre-SET domain
IPR016177 DNA-binding domain

PFAM

PF00856
PF01429
PF00567
PF05033

PRINTS

PIRSF

SMART

SM00317
SM00391
SM00333
SM00468
SM00508

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q15047