InnateDB Protein
|
IDBP-128403.6
|
Last Modified
|
2014-10-13 [Report errors or provide feedback]
|
Gene Symbol
|
Rpa3
|
Protein Name
|
replication protein A3
|
Synonyms
|
14kDa; C330026P08Rik;
|
Species
|
Mus musculus
|
Ensembl Protein
|
ENSMUSP00000012627
|
InnateDB Gene
|
IDBG-128401 (Rpa3)
|
Protein Structure
|
|
Function |
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER), probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (By similarity). {ECO:0000250}.
|
Subcellular Localization |
Nucleus {ECO:0000250}.
|
Disease Associations |
|
Tissue Specificity |
|
Comments |
|
Number of Interactions
|
This gene and/or its encoded proteins are associated with 5 experimentally validated interaction(s) in this database.
They are also associated with 35 interaction(s) predicted by orthology.
Experimentally validated |
Total |
5
[view]
|
Protein-Protein |
5
[view]
|
Protein-DNA |
0
|
Protein-RNA |
0
|
DNA-DNA |
0
|
RNA-RNA |
0
|
DNA-RNA |
0
|
|
Predicted by orthology |
Total |
35 [view]
|
|
|
Molecular Function |
|
Biological Process |
|
Cellular Component |
|
PDB ID |
MGI:1915490
|
InterPro |
IPR012340
Nucleic acid-binding, OB-fold
IPR013970
Replication factor A protein 3
|
PFAM |
PF08661
|
PRINTS |
|
PIRSF |
|
SMART |
|
TIGRFAMs |
|
Modification |
|
SwissProt |
Q9CQ71
|
PhosphoSite |
PhosphoSite-
|
TrEMBL |
|
UniProt Splice Variant |
|
Entrez Gene |
68240
|
UniGene |
|
RefSeq |
NP_080908
|
MGI ID |
|
MGI Symbol |
Rpa3
|
OMIM |
|
CCDS |
CCDS19909
|
HPRD |
|
IMGT |
|
EMBL |
AK005285
AK009916
AK021228
BC028489
|
GenPept |
AAH28489
BAB23932
BAB26583
BAB32338
|
|
|