InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rnf31

Summary

InnateDB Protein

IDBP-164241.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rnf31

Protein Name

ring finger protein 31

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000019443

InnateDB Gene

IDBG-164239 (Rnf31)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF- induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:14678832}.

Disease Associations

Tissue Specificity

Widely expressed (at protein level). Not expressed in heart. {ECO:0000269PubMed:14678832}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 23 experimentally validated interaction(s) in this database.
They are also associated with 32 interaction(s) predicted by orthology.

Experimentally validated
Total	23 [view]
Protein-Protein	21 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	32 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0043130	ubiquitin binding

Biological Process

GO:0000209	protein polyubiquitination
GO:0016567	protein ubiquitination
GO:0023035	CD40 signaling pathway
GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0050852	T cell receptor signaling pathway
GO:0051092	positive regulation of NF-kappaB transcription factor activity
GO:0060546	negative regulation of necroptotic process
GO:0097039	protein linear polyubiquitination

Cellular Component

GO:0005829	cytosol
GO:0009898	cytoplasmic side of plasma membrane
GO:0035631	CD40 receptor complex
GO:0071797	LUBAC complex

Protein Structure and Domains

PDB ID

MGI:1934704

InterPro

IPR001876 Zinc finger, RanBP2-type
IPR002867 Zinc finger, C6HC-type
IPR009060 UBA-like
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote
IPR018997 PUB domain

PFAM

PF00641
PF01485
PF09409

PRINTS

PIRSF

SMART

SM00547
SM00647
SM00165

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q924T7