InnateDB Protein
|
IDBP-204806.6
|
Last Modified
|
2014-10-13 [Report errors or provide feedback]
|
Gene Symbol
|
Astl
|
Protein Name
|
astacin-like metalloendopeptidase (M12 family)
|
Synonyms
|
|
Species
|
Mus musculus
|
Ensembl Protein
|
ENSMUSP00000087102
|
InnateDB Gene
|
IDBG-204802 (Astl)
|
Protein Structure
|
|
Function |
Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding. {ECO:0000269PubMed:22206759, ECO:0000269PubMed:22472438}.
|
Subcellular Localization |
Cytoplasm. Cell membrane. Cytoplasmic granule. Cytoplasmic vesicle, secretory vesicle. Note=Secretory granules. Localizes to the peripheral cortical granules of ovulated eggs. Probably exocytosed from cortical granules during post-fertilization. Detected throughout the ooplasm of germinal vesicle stage oocytes in early bilaminar secondary follicles at postnatal (PN) day 3. Detected in the microvillar domain of the oolemma in arrested ovulated secondary oocytes and in the first polar body prior to fertilization. Upon fertilization, detected in the perivitelline space (PVS) and occasionally on the oolemma in 2-cell through morulae stages. Colocalizes with SPACA3 at the microvillar domain of the oolemma and in the perivitelline space (PVS).
|
Disease Associations |
|
Tissue Specificity |
Ovary-specific. Expressed in secondary, antral and Graafian follicle oocytes. Expressed in the egg cells. Not detected in two-cell embryos. Not detected in naked oocytes, oocytes in primordial or unilaminar primary follicles, or in any other ovarian cells at pre-pubertal, pubertal or adult stages (at protein level). Ovary-specific. {ECO:0000269PubMed:22206759, ECO:0000269PubMed:22472438}.
|
Comments |
|
Number of Interactions
|
This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
|
Molecular Function |
|
Biological Process |
|
Cellular Component |
|
PDB ID |
MGI:3046414
|
InterPro |
IPR001506
Peptidase M12A, astacin
IPR006026
Peptidase, metallopeptidase
|
PFAM |
PF01400
|
PRINTS |
PR00480
|
PIRSF |
|
SMART |
SM00235
|
TIGRFAMs |
|
Modification |
|
SwissProt |
Q6HA09
|
PhosphoSite |
PhosphoSite-Q6HA09
|
TrEMBL |
A2ARG6
|
UniProt Splice Variant |
|
Entrez Gene |
215095
|
UniGene |
Mm.481984
|
RefSeq |
NP_001277932
|
MGI ID |
|
MGI Symbol |
Astl
|
OMIM |
|
CCDS |
CCDS71138
|
HPRD |
|
IMGT |
|
EMBL |
AJ537599
AK033037
AL731836
AL845368
BC064729
FJ187790
FJ187791
FJ187792
FJ187793
FJ187794
FJ187795
|
GenPept |
AAH64729
ACN71160
ACN71161
ACN71162
ACN71163
ACN71164
ACN71165
BAC28134
CAD61264
CAM13879
CAM13880
CAM17447
CAM17448
|
|
|