InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: POLR2B

Summary

InnateDB Protein

IDBP-20532.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

POLR2B

Protein Name

polymerase (RNA) II (DNA directed) polypeptide B, 140kDa

Synonyms

hRPB140; hsRPB2; POL2RB; RPB2;

Species

Homo sapiens

Ensembl Protein

ENSP00000370625

InnateDB Gene

IDBG-20530 (POLR2B)

Protein Structure

UniProt Annotation

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:9852112}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 94 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	94 [view]
Protein-Protein	93 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003899	DNA-directed RNA polymerase activity
GO:0005515	protein binding
GO:0032549	ribonucleoside binding
GO:0044822	poly(A) RNA binding
GO:0046872	metal ion binding

Biological Process

GO:0000398	mRNA splicing, via spliceosome
GO:0006281	DNA repair
GO:0006283	transcription-coupled nucleotide-excision repair
GO:0006289	nucleotide-excision repair
GO:0006351	transcription, DNA-templated
GO:0006366	transcription from RNA polymerase II promoter
GO:0006367	transcription initiation from RNA polymerase II promoter
GO:0006368	transcription elongation from RNA polymerase II promoter
GO:0006370	7-methylguanosine mRNA capping
GO:0008380	RNA splicing
GO:0010467	gene expression
GO:0016032	viral process
GO:0050434	positive regulation of viral transcription

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005665	DNA-directed RNA polymerase II, core complex
GO:0016020	membrane

Protein Structure and Domains

PDB ID

InterPro

IPR007120 DNA-directed RNA polymerase, subunit 2, domain 6
IPR007641 RNA polymerase Rpb2, domain 7
IPR007642 RNA polymerase Rpb2, domain 2
IPR007644 RNA polymerase, beta subunit, protrusion
IPR007645 RNA polymerase Rpb2, domain 3
IPR007646 RNA polymerase Rpb2, domain 4
IPR007647 RNA polymerase Rpb2, domain 5

PFAM