InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Pcna

Summary

InnateDB Protein

IDBP-207310.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Pcna

Protein Name

proliferating cell nuclear antigen

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000028817

InnateDB Gene

IDBG-207308 (Pcna)

Protein Structure

UniProt Annotation

Function

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:24115439}. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 36 experimentally validated interaction(s) in this database.

Experimentally validated
Total	36 [view]
Protein-Protein	35 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0005515	protein binding
GO:0030337	DNA polymerase processivity factor activity

Biological Process

GO:0000077	DNA damage checkpoint
GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006272	leading strand elongation
GO:0006275	regulation of DNA replication
GO:0006287	base-excision repair, gap-filling
GO:0006298	mismatch repair
GO:0019985	translesion synthesis
GO:0032077	positive regulation of deoxyribonuclease activity

Cellular Component

GO:0000307	cyclin-dependent protein kinase holoenzyme complex
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005652	nuclear lamina
GO:0005657	replication fork
GO:0005813	centrosome
GO:0030896	checkpoint clamp complex
GO:0043626	PCNA complex
GO:0070557	PCNA-p21 complex

Protein Structure and Domains

PDB ID

MGI:97503

InterPro

IPR000730 Proliferating cell nuclear antigen, PCNA
IPR007268 Rad9/Ddc1
IPR022648 Proliferating cell nuclear antigen, PCNA, N-terminal
IPR022649 Proliferating cell nuclear antigen, PCNA, C-terminal

PFAM

PF04139
PF00705
PF02747

PRINTS

PR00339

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P17918