InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PRMT5

Summary

InnateDB Protein

IDBP-235305.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PRMT5

Protein Name

protein arginine methyltransferase 5

Synonyms

HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs;

Species

Homo sapiens

Ensembl Protein

ENSP00000380583

InnateDB Gene

IDBG-2920 (PRMT5)

Protein Structure

UniProt Annotation

Function

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation. Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9. Methylates and regulates SRGAP2 which is involved in cell migration and differentiation. Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter. {ECO:0000269PubMed:10531356, ECO:0000269PubMed:11152681, ECO:0000269PubMed:11747828, ECO:0000269PubMed:12411503, ECO:0000269PubMed:15737618, ECO:0000269PubMed:17709427, ECO:0000269PubMed:20159986, ECO:0000269PubMed:20810653, ECO:0000269PubMed:21258366, ECO:0000269PubMed:21917714, ECO:0000269PubMed:22269951}.

Subcellular Localization

Cytoplasm. Nucleus.

Disease Associations

Tissue Specificity

Ubiquitous.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 217 experimentally validated interaction(s) in this database.
They are also associated with 21 interaction(s) predicted by orthology.

Experimentally validated
Total	217 [view]
Protein-Protein	211 [view]
Protein-DNA	6 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	21 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001046	core promoter sequence-specific DNA binding
GO:0003714	transcription corepressor activity
GO:0005515	protein binding
GO:0008168	methyltransferase activity
GO:0008469	histone-arginine N-methyltransferase activity
GO:0035243	protein-arginine omega-N symmetric methyltransferase activity
GO:0043021	ribonucleoprotein complex binding

Biological Process

GO:0000387	spliceosomal snRNP assembly
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0006479	protein methylation
GO:0007088	regulation of mitosis
GO:0008283	cell proliferation
GO:0010467	gene expression
GO:0016070	RNA metabolic process
GO:0018216	peptidyl-arginine methylation
GO:0019918	peptidyl-arginine methylation, to symmetrical-dimethyl arginine
GO:0032922	circadian regulation of gene expression
GO:0034660	ncRNA metabolic process
GO:0035246	peptidyl-arginine N-methylation
GO:0042118	endothelial cell activation
GO:0043985	histone H4-R3 methylation

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0034709	methylosome

Protein Structure and Domains

PDB ID

InterPro

IPR007857 Protein arginine N-methyltransferase PRMT5
IPR025799 Protein arginine N-methyltransferase
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase-like

PFAM

PF05185

PRINTS

PIRSF

PIRSF015894

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

O14744