InnateDB Protein
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IDBP-255401.6
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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Apex2
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Protein Name
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apurinic/apyrimidinic endonuclease 2
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Synonyms
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ape2; C430040P13Rik;
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Species
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Mus musculus
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Ensembl Protein
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ENSMUSP00000108347
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InnateDB Gene
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IDBG-178133 (Apex2)
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Protein Structure
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Function |
Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. {ECO:0000269PubMed:12573260, ECO:0000269PubMed:15319281, ECO:0000269PubMed:18025127, ECO:0000269PubMed:19556307}.
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Subcellular Localization |
Nucleus. Cytoplasm. Mitochondrion {ECO:0000305}. Note=Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
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Disease Associations |
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Tissue Specificity |
Expressed in lymphocytes, thymocytes and splenocytes (at protein level). Highly expressed in the thymus and weakly expressed in the bone marrow, spleen, eye, kidney, lung, brain and uterus. {ECO:0000269PubMed:12573260, ECO:0000269PubMed:15319281}.
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 11 interaction(s) predicted by orthology.
Experimentally validated |
Total |
1
[view]
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Protein-Protein |
1
[view]
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Protein-DNA |
0
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Predicted by orthology |
Total |
11 [view]
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Molecular Function |
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Biological Process |
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Cellular Component |
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PDB ID |
MGI:1924872
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InterPro |
IPR005135
Endonuclease/exonuclease/phosphatase
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PFAM |
PF03372
PF14529
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PRINTS |
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PIRSF |
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SMART |
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TIGRFAMs |
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Modification |
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SwissProt |
Q68G58
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PhosphoSite |
PhosphoSite-Q68G58
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TrEMBL |
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UniProt Splice Variant |
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Entrez Gene |
77622
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UniGene |
Mm.453804
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RefSeq |
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MGI ID |
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MGI Symbol |
Apex2
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OMIM |
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CCDS |
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HPRD |
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IMGT |
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EMBL |
AB072498
AB085235
AK021248
AK040145
AK050858
AK080916
AK081677
AK088918
BC026769
BC078633
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GenPept |
AAH26769
AAH78633
BAB32346
BAB88654
BAC11807
BAC30522
BAC34436
BAC38077
BAC38287
BAC40652
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