InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CHMP4C

Summary

InnateDB Protein

IDBP-26896.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CHMP4C

Protein Name

charged multivesicular body protein 4C

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000297265

InnateDB Gene

IDBG-26894 (CHMP4C)

Protein Structure

UniProt Annotation

Function

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: upon phosphorylation by AURKB, together with ZFYVE19/ANCHR, retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ANCHR and VPS4 and subsequent abscission (PubMed:22422861, PubMed:24814515). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV- 1 p6- and p9-dependent virus release. {ECO:0000269PubMed:14505569, ECO:0000269PubMed:14505570, ECO:0000269PubMed:14519844, ECO:0000269PubMed:22422861, ECO:0000269PubMed:24814515}.

Subcellular Localization

Cytoplasm, cytosol. Late endosome membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Midbody. Note=Localizes to the midbody during cytokinesis. Phosphorylation at Ser-210 by AURKB triggers localization to Flemming body (PubMed:22422861). {ECO:0000269PubMed:22422861}.

Disease Associations

Tissue Specificity

Expressed in heart, spleen and kidney. {ECO:0000269PubMed:14583093, ECO:0000269PubMed:14678797}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 13 experimentally validated interaction(s) in this database.

Experimentally validated
Total	13 [view]
Protein-Protein	13 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0042803	protein homodimerization activity

Biological Process

GO:0007034	vacuolar transport
GO:0009838	abscission
GO:0015031	protein transport
GO:0016032	viral process
GO:0016197	endosomal transport
GO:0019058	viral life cycle
GO:0031565	cytokinesis checkpoint
GO:0032466	negative regulation of cytokinesis
GO:0050792	regulation of viral process
GO:0061024	membrane organization
GO:1902188	positive regulation of viral release from host cell