InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: GEN1

Summary

InnateDB Protein

IDBP-31448.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

GEN1

Protein Name

Gen homolog 1, endonuclease (Drosophila)

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000318977

InnateDB Gene

IDBG-31446 (GEN1)

Protein Structure

UniProt Annotation

Function

Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. {ECO:0000269PubMed:19020614}.

Subcellular Localization

Nucleus {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 2 experimentally validated interaction(s) in this database.

Experimentally validated
Total	2 [view]
Protein-Protein	2 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003824	catalytic activity
GO:0004518	nuclease activity
GO:0008821	crossover junction endodeoxyribonuclease activity
GO:0046872	metal ion binding

Biological Process

GO:0000724	double-strand break repair via homologous recombination
GO:0000737	DNA catabolic process, endonucleolytic
GO:0006281	DNA repair
GO:0010824	regulation of centrosome duplication
GO:0071139	resolution of recombination intermediates
GO:0071140	resolution of mitotic recombination intermediates
GO:0090267	positive regulation of mitotic cell cycle spindle assembly checkpoint
GO:0090305	nucleic acid phosphodiester bond hydrolysis

Cellular Component

GO:0005634	nucleus
GO:0005813	centrosome
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR006085 XPG N-terminal
IPR006086 XPG-I domain
IPR008918 Helix-hairpin-helix motif, class 2
IPR020045 5\'-3\' exonuclease, C-terminal domain
IPR029060 PIN domain-like

PFAM

PF00752
PF00867
PF01367
PF09293

PRINTS

PIRSF

SMART

SM00485
SM00484
SM00279

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q17RS7