InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: AMFR

Summary

InnateDB Protein

IDBP-31787.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

AMFR

Protein Name

autocrine motility factor receptor

Synonyms

GP78; RNF45;

Species

Homo sapiens

Ensembl Protein

ENSP00000290649

InnateDB Gene

IDBG-31785 (AMFR)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor. {ECO:0000269PubMed:10456327, ECO:0000269PubMed:11724934, ECO:0000269PubMed:16168377, ECO:0000269PubMed:19103148}.

Subcellular Localization

Endoplasmic reticulum membrane {ECO:0000269PubMed:11724934}; Multi-pass membrane protein {ECO:0000269PubMed:11724934}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 116 experimentally validated interaction(s) in this database.
They are also associated with 8 interaction(s) predicted by orthology.

Experimentally validated
Total	116 [view]
Protein-Protein	115 [view]
Protein-DNA	0
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	8 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0004872	receptor activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0046872	metal ion binding

Biological Process

GO:0000209	protein polyubiquitination
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0006928	cellular component movement
GO:0007165	signal transduction
GO:0007568	aging
GO:0007611	learning or memory
GO:0030433	ER-associated ubiquitin-dependent protein catabolic process
GO:0030968	endoplasmic reticulum unfolded protein response
GO:0045087	innate immune response (InnateDB)
GO:0051259	protein oligomerization

Cellular Component

GO:0000836	Hrd1p ubiquitin ligase complex
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005789	endoplasmic reticulum membrane
GO:0016020	membrane
GO:0016021	integral component of membrane
GO:0030176	integral component of endoplasmic reticulum membrane
GO:0030425	dendrite
GO:0030426	growth cone
GO:0043025	neuronal cell body
GO:0043234	protein complex
GO:0048471	perinuclear region of cytoplasm