InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PKN1

Summary

InnateDB Protein

IDBP-33270.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PKN1

Protein Name

protein kinase N1

Synonyms

DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1;

Species

Homo sapiens

Ensembl Protein

ENSP00000242783

InnateDB Gene

IDBG-33268 (PKN1)

Protein Structure

UniProt Annotation

Function

PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser- 159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro. {ECO:0000269PubMed:11104762, ECO:0000269PubMed:12514133, ECO:0000269PubMed:17332740, ECO:0000269PubMed:18066052, ECO:0000269PubMed:20188095, ECO:0000269PubMed:21754995, ECO:0000269PubMed:24248594, ECO:0000269PubMed:8557118, ECO:0000269PubMed:8621664, ECO:0000269PubMed:9175763}.

Subcellular Localization

Cytoplasm. Nucleus. Endosome. Cell membrane; Peripheral membrane protein. Cleavage furrow. Midbody. Note=Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.

Disease Associations

Tissue Specificity

Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells. {ECO:0000269PubMed:21754995}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 65 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	65 [view]
Protein-Protein	63 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003682	chromatin binding
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004697	protein kinase C activity
GO:0004713	protein tyrosine kinase activity
GO:0005080	protein kinase C binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0017049	GTP-Rho binding
GO:0030374	ligand-dependent nuclear receptor transcription coactivator activity
GO:0035402	histone kinase activity (H3-T11 specific)
GO:0042393	histone binding
GO:0042826	histone deacetylase binding
GO:0048365	Rac GTPase binding
GO:0050681	androgen receptor binding

Biological Process

GO:0006351	transcription, DNA-templated
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0006468	protein phosphorylation
GO:0007165	signal transduction
GO:0007257	activation of JUN kinase activity
GO:0010631	epithelial cell migration
GO:0035407	histone H3-T11 phosphorylation
GO:0045087	innate immune response (InnateDB)
GO:2000145	regulation of cell motility

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005768	endosome
GO:0005886	plasma membrane
GO:0030496	midbody
GO:0032154	cleavage furrow

Protein Structure and Domains

PDB ID

InterPro

IPR000008 C2 domain
IPR000719 Protein kinase domain
IPR000961 AGC-kinase, C-terminal
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR011072 HR1 rho-binding repeat
IPR017892 Protein kinase, C-terminal
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00168
PF00069
PF07714
PF02185
PF00433

PRINTS

PR00360
PR00109

PIRSF

SMART

SM00239
SM00133
SM00220
SM00742
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt