InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: DNMT3A

Summary

InnateDB Protein

IDBP-34447.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

DNMT3A

Protein Name

DNA (cytosine-5-)-methyltransferase 3 alpha

Synonyms

DNMT3A2; M.HsaIIIA; TBRS;

Species

Homo sapiens

Ensembl Protein

ENSP00000264709

InnateDB Gene

IDBG-34441 (DNMT3A)

Protein Structure

UniProt Annotation

Function

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. {ECO:0000269PubMed:16357870}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:12145218}. Cytoplasm {ECO:0000269PubMed:12145218}. Note=Accumulates in the major satellite repeats at pericentric heterochromatin. {ECO:0000250}.

Disease Associations

Tissue Specificity

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. {ECO:0000269PubMed:10325416}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 90 experimentally validated interaction(s) in this database.
They are also associated with 8 interaction(s) predicted by orthology.

Experimentally validated
Total	90 [view]
Protein-Protein	86 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	8 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003886	DNA (cytosine-5-)-methyltransferase activity
GO:0005515	protein binding
GO:0008168	methyltransferase activity
GO:0045322	unmethylated CpG binding
GO:0046872	metal ion binding
GO:0051718	DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006306	DNA methylation
GO:0006346	methylation-dependent chromatin silencing
GO:0006349	regulation of gene expression by genetic imprinting
GO:0007283	spermatogenesis
GO:0010424	DNA methylation on cytosine within a CG sequence
GO:0043045	DNA methylation involved in embryo development
GO:0043046	DNA methylation involved in gamete generation
GO:0044027	hypermethylation of CpG island
GO:0046498	S-adenosylhomocysteine metabolic process
GO:0046499	S-adenosylmethioninamine metabolic process
GO:0071230	cellular response to amino acid stimulus
GO:0090116	C-5 methylation of cytosine

Cellular Component

GO:0000775	chromosome, centromeric region
GO:0000791	euchromatin
GO:0000792	heterochromatin
GO:0005634	nucleus
GO:0005720	nuclear heterochromatin
GO:0005737	cytoplasm
GO:0016363	nuclear matrix

Protein Structure and Domains

PDB ID

InterPro

IPR000313 PWWP domain
IPR001525 C-5 cytosine methyltransferase
IPR011011 Zinc finger, FYVE/PHD-type
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase-like

PFAM

PF00855
PF00145

PRINTS

PR00105

PIRSF

SMART

SM00293

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt