InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: NAE1

Summary

InnateDB Protein

IDBP-35589.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NAE1

Protein Name

NEDD8 activating enzyme E1 subunit 1

Synonyms

A-116A10.1; APPBP1; ula-1;

Species

Homo sapiens

Ensembl Protein

ENSP00000290810

InnateDB Gene

IDBG-35583 (NAE1)

Protein Structure

UniProt Annotation

Function

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. {ECO:0000269PubMed:10207026, ECO:0000269PubMed:10722740, ECO:0000269PubMed:12740388}.

Subcellular Localization

Cell membrane {ECO:0000269PubMed:14557245}. Note=Colocalizes with APP in lipid rafts.

Disease Associations

Tissue Specificity

Ubiquitous in fetal tissues. Expressed throughout the adult brain. {ECO:0000269PubMed:8626687}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 44 experimentally validated interaction(s) in this database.

Experimentally validated
Total	44 [view]
Protein-Protein	44 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003824	catalytic activity
GO:0005515	protein binding
GO:0019781	NEDD8 activating enzyme activity
GO:0031625	ubiquitin protein ligase binding
GO:0046982	protein heterodimerization activity

Biological Process

GO:0007165	signal transduction
GO:0033314	mitotic DNA replication checkpoint
GO:0042981	regulation of apoptotic process
GO:0043523	regulation of neuron apoptotic process
GO:0045116	protein neddylation
GO:0051402	neuron apoptotic process