InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RACGAP1

Summary

InnateDB Protein

IDBP-374726.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RACGAP1

Protein Name

Rac GTPase activating protein 1

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000404190

InnateDB Gene

IDBG-32209 (RACGAP1)

Protein Structure

UniProt Annotation

Function

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells. {ECO:0000269PubMed:10979956, ECO:0000269PubMed:11085985, ECO:0000269PubMed:11278976, ECO:0000269PubMed:11782313, ECO:0000269PubMed:14729465, ECO:0000269PubMed:15642749, ECO:0000269PubMed:16103226, ECO:0000269PubMed:16129829, ECO:0000269PubMed:16236794, ECO:0000269PubMed:19468300, ECO:0000269PubMed:19468302, ECO:0000269PubMed:23235882, ECO:0000269PubMed:9497316}.

Subcellular Localization

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavage furrow. Midbody. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with RND2 in Golgi- derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase. {ECO:0000250}.

Disease Associations

Tissue Specificity

Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase. {ECO:0000269PubMed:10979956, ECO:0000269PubMed:11278976, ECO:0000269PubMed:12590651, ECO:0000269PubMed:9497316}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 50 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	50 [view]
Protein-Protein	50 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005096	GTPase activator activity
GO:0005515	protein binding
GO:0005547	phosphatidylinositol-3,4,5-trisphosphate binding
GO:0008017	microtubule binding
GO:0019901	protein kinase binding
GO:0043014	alpha-tubulin binding
GO:0043015	gamma-tubulin binding
GO:0046872	metal ion binding
GO:0048487	beta-tubulin binding

Biological Process

GO:0000281	mitotic cytokinesis
GO:0000915	cytokinesis, actomyosin contractile ring assembly
GO:0007018	microtubule-based movement
GO:0007108	cytokinesis, initiation of separation
GO:0007165	signal transduction
GO:0007264	small GTPase mediated signal transduction
GO:0007283	spermatogenesis
GO:0007405	neuroblast proliferation
GO:0007596	blood coagulation
GO:0008272	sulfate transport
GO:0009790	embryo development
GO:0019886	antigen processing and presentation of exogenous peptide antigen via MHC class II
GO:0032467	positive regulation of cytokinesis
GO:0035556	intracellular signal transduction
GO:0038032	termination of G-protein coupled receptor signaling pathway
GO:0043547	positive regulation of GTPase activity
GO:0051056	regulation of small GTPase mediated signal transduction
GO:0051256	spindle midzone assembly involved in mitosis
GO:0051988	regulation of attachment of spindle microtubules to kinetochore

Cellular Component

GO:0001669	acrosomal vesicle
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005829	cytosol
GO:0005874	microtubule
GO:0030496	midbody
GO:0031234	extrinsic component of cytoplasmic side of plasma membrane
GO:0032154	cleavage furrow
GO:0051233	spindle midzone
GO:0070062	extracellular vesicular exosome
GO:0072686	mitotic spindle
GO:0097149	centralspindlin complex

Protein Structure and Domains

PDB ID

InterPro

IPR000198 Rho GTPase-activating protein domain
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
IPR008936 Rho GTPase activation protein
IPR016137 Regulator of G protein signalling superfamily

PFAM

PF00620
PF00130

PRINTS

PIRSF

SMART

SM00324
SM00109
SM00315

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9H0H5