InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RHOA

Summary

InnateDB Protein

IDBP-377198.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RHOA

Protein Name

ras homolog gene family, member A

Synonyms

ARH12; ARHA; RHO12; RHOH12;

Species

Homo sapiens

Ensembl Protein

ENSP00000400175

InnateDB Gene

IDBG-34732 (RHOA)

Protein Structure

UniProt Annotation

Function

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA- DIAPH1 signaling pathway plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. {ECO:0000269PubMed:12900402, ECO:0000269PubMed:16103226, ECO:0000269PubMed:16236794, ECO:0000269PubMed:19934221, ECO:0000269PubMed:20937854, ECO:0000269PubMed:20974804, ECO:0000269PubMed:8910519, ECO:0000269PubMed:9121475}.

Subcellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex. Midbody. Cell projection, lamellipodium {ECO:0000250}. Note=Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 171 experimentally validated interaction(s) in this database.
They are also associated with 12 interaction(s) predicted by orthology.

Experimentally validated
Total	171 [view]
Protein-Protein	169 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	2 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	12 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003924	GTPase activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0017022	myosin binding

Biological Process

GO:0006184	GTP catabolic process
GO:0007165	signal transduction
GO:0007179	transforming growth factor beta receptor signaling pathway
GO:0007264	small GTPase mediated signal transduction
GO:0007266	Rho protein signal transduction
GO:0007411	axon guidance
GO:0007596	blood coagulation
GO:0015031	protein transport
GO:0016032	viral process
GO:0021762	substantia nigra development
GO:0030036	actin cytoskeleton organization
GO:0030168	platelet activation
GO:0030334	regulation of cell migration
GO:0032467	positive regulation of cytokinesis
GO:0033688	regulation of osteoblast proliferation
GO:0036089	cleavage furrow formation
GO:0042346	positive regulation of NF-kappaB import into nucleus
GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043297	apical junction assembly
GO:0043931	ossification involved in bone maturation
GO:0045666	positive regulation of neuron differentiation
GO:0048011	neurotrophin TRK receptor signaling pathway
GO:0048015	phosphatidylinositol-mediated signaling
GO:0050770	regulation of axonogenesis
GO:0050771	negative regulation of axonogenesis
GO:0050772	positive regulation of axonogenesis
GO:0051056	regulation of small GTPase mediated signal transduction
GO:0051496	positive regulation of stress fiber assembly
GO:0061383	trabecula morphogenesis
GO:0090307	spindle assembly involved in mitosis

Cellular Component

GO:0005622	intracellular
GO:0005829	cytosol
GO:0005856	cytoskeleton
GO:0005886	plasma membrane
GO:0005938	cell cortex
GO:0016020	membrane
GO:0030027	lamellipodium
GO:0030054	cell junction
GO:0030496	midbody
GO:0032154	cleavage furrow
GO:0043296	apical junction complex
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR000795 Elongation factor, GTP-binding domain
IPR001806 Small GTPase superfamily
IPR003578 Small GTPase superfamily, Rho type
IPR003579 Small GTPase superfamily, Rab type
IPR005225 Small GTP-binding protein domain
IPR006689 Small GTPase superfamily, ARF/SAR type
IPR013684 Mitochondrial Rho-like
IPR020849 Small GTPase superfamily, Ras type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00009
PF00071
PF00025
PF08477

PRINTS

PR00315
PR00449
PR00328

PIRSF

SMART

SM00174
SM00175
SM00173

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt