InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CHST4

Summary

InnateDB Protein

IDBP-40960.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CHST4

Protein Name

carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4

Synonyms

GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST;

Species

Homo sapiens

Ensembl Protein

ENSP00000341206

InnateDB Gene

IDBG-40958 (CHST4)

Protein Structure

UniProt Annotation

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2- branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1- 3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location. {ECO:0000269PubMed:10330415}.

Subcellular Localization

Golgi apparatus membrane {ECO:0000269PubMed:12855678}; Single-pass type II membrane protein {ECO:0000269PubMed:12855678}.

Disease Associations

Tissue Specificity

Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma. {ECO:0000269PubMed:10330415, ECO:0000269PubMed:11310842, ECO:0000269PubMed:12107080}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001517	N-acetylglucosamine 6-O-sulfotransferase activity
GO:0008146	sulfotransferase activity

Biological Process

GO:0005975	carbohydrate metabolic process
GO:0006044	N-acetylglucosamine metabolic process
GO:0006477	protein sulfation
GO:0006790	sulfur compound metabolic process
GO:0006928	cellular component movement
GO:0006954	inflammatory response
GO:0006955	immune response
GO:0007155	cell adhesion
GO:0007267	cell-cell signaling
GO:0050901	leukocyte tethering or rolling