InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: DDB1

Summary

InnateDB Protein

IDBP-49966.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

DDB1

Protein Name

damage-specific DNA binding protein 1, 127kDa

Synonyms

DDBA; UV-DDB1; XAP1; XPCE; XPE; XPE-BF;

Species

Homo sapiens

Ensembl Protein

ENSP00000301764

InnateDB Gene

IDBG-49964 (DDB1)

Protein Structure

UniProt Annotation

Function

Required for DNA repair. Binds to DDB2 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex). The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin- protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. {ECO:0000269PubMed:12732143, ECO:0000269PubMed:14739464, ECO:0000269PubMed:15448697, ECO:0000269PubMed:15882621, ECO:0000269PubMed:16260596, ECO:0000269PubMed:16407242, ECO:0000269PubMed:16407252, ECO:0000269PubMed:16473935, ECO:0000269PubMed:16482215, ECO:0000269PubMed:16678110, ECO:0000269PubMed:16940174, ECO:0000269PubMed:17041588, ECO:0000269PubMed:17079684, ECO:0000269PubMed:18332868, ECO:0000269PubMed:18381890, ECO:0000269PubMed:18593899}.

Subcellular Localization

Cytoplasm. Nucleus. Note=Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 290 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.

Experimentally validated
Total	290 [view]
Protein-Protein	288 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	4 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0005515	protein binding

Biological Process

GO:0000718	nucleotide-excision repair, DNA damage removal
GO:0006281	DNA repair
GO:0006289	nucleotide-excision repair
GO:0016032	viral process
GO:0016055	Wnt signaling pathway
GO:0035518	histone H2A monoubiquitination
GO:0042787	protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0043066	negative regulation of apoptotic process
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0070914	UV-damage excision repair
GO:1901990	regulation of mitotic cell cycle phase transition

Cellular Component

GO:0005615	extracellular space
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0031464	Cul4A-RING E3 ubiquitin ligase complex
GO:0031465	Cul4B-RING E3 ubiquitin ligase complex
GO:0070062	extracellular vesicular exosome
GO:0080008	Cul4-RING E3 ubiquitin ligase complex