InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ARF1

Summary

InnateDB Protein

IDBP-583154.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ARF1

Protein Name

ADP-ribosylation factor 1

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000442980

InnateDB Gene

IDBG-107128 (ARF1)

Protein Structure

UniProt Annotation

Function

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

Subcellular Localization

Golgi apparatus {ECO:0000269PubMed:17555535}. Cytoplasm, perinuclear region {ECO:0000269PubMed:17555535}. Cell junction, synapse, synaptosome {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 57 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	57 [view]
Protein-Protein	56 [view]
Protein-DNA	0
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003924	GTPase activity
GO:0004871	signal transducer activity
GO:0005057	receptor signaling protein activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0019001	guanyl nucleotide binding
GO:0031683	G-protein beta/gamma-subunit complex binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0002090	regulation of receptor internalization
GO:0006184	GTP catabolic process
GO:0006644	phospholipid metabolic process
GO:0006661	phosphatidylinositol biosynthetic process
GO:0006878	cellular copper ion homeostasis
GO:0006886	intracellular protein transport
GO:0006890	retrograde vesicle-mediated transport, Golgi to ER
GO:0006892	post-Golgi vesicle-mediated transport
GO:0007186	G-protein coupled receptor signaling pathway
GO:0007264	small GTPase mediated signal transduction
GO:0015031	protein transport
GO:0016032	viral process
GO:0019886	antigen processing and presentation of exogenous peptide antigen via MHC class II
GO:0034315	regulation of Arp2/3 complex-mediated actin nucleation
GO:0044281	small molecule metabolic process
GO:0048205	COPI coating of Golgi vesicle
GO:0050690	regulation of defense response to virus by virus
GO:0060292	long term synaptic depression
GO:0061024	membrane organization
GO:0097061	dendritic spine organization

Cellular Component

GO:0000139	Golgi membrane
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0014069	postsynaptic density
GO:0030054	cell junction
GO:0043005	neuron projection
GO:0045211	postsynaptic membrane
GO:0048471	perinuclear region of cytoplasm
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR001019 Guanine nucleotide binding protein (G-protein), alpha subunit
IPR001806 Small GTPase superfamily
IPR003579 Small GTPase superfamily, Rab type
IPR005225 Small GTP-binding protein domain
IPR006687 Small GTPase superfamily, SAR1-type
IPR006689 Small GTPase superfamily, ARF/SAR type
IPR006762 Gtr1/RagA G protein
IPR013684 Mitochondrial Rho-like
IPR019009 Signal recognition particle receptor, beta subunit
IPR024156 Small GTPase superfamily, ARF type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00503
PF00071
PF00025
PF04670
PF08477
PF09439

PRINTS

PR00318
PR00449
PR00328

PIRSF

SMART

SM00275
SM00175
SM00178
SM00177

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt