InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ARF6

Summary

InnateDB Protein

IDBP-5866.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ARF6

Protein Name

ADP-ribosylation factor 6

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000298316

InnateDB Gene

IDBG-5864 (ARF6)

Protein Structure

UniProt Annotation

Function

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. {ECO:0000269PubMed:11266366, ECO:0000269PubMed:14978216, ECO:0000269PubMed:16099990, ECO:0000269PubMed:16737952, ECO:0000269PubMed:18400762, ECO:0000269PubMed:21170023, ECO:0000269PubMed:7589240}.

Subcellular Localization

Golgi apparatus. Cell membrane; Lipid- anchor. Endosome membrane; Lipid-anchor. Recycling endosome membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}. Cell projection, filopodium membrane; Lipid-anchor. Midbody {ECO:0000250}. Cytoplasm {ECO:0000250}. Cleavage furrow {ECO:0000250}. Note=Distributed throughout the cytoplasm during metaphase. Transiently detected at the ingressing cleavage furrow during mitotic cytokinesis. Recruited to the midbody at later stages of cytokinesis; this requires interaction with KIF23 (By similarity). Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles. {ECO:0000250}.

Disease Associations

Tissue Specificity

Ubiquitous, with higher levels in heart, substantia nigra, and kidney. {ECO:0000269PubMed:14659046}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 69 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.

Experimentally validated
Total	69 [view]
Protein-Protein	69 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	4 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003924	GTPase activity
GO:0004871	signal transducer activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0019001	guanyl nucleotide binding
GO:0031683	G-protein beta/gamma-subunit complex binding
GO:0031996	thioesterase binding

Biological Process

GO:0001889	liver development
GO:0006184	GTP catabolic process
GO:0006886	intracellular protein transport
GO:0006928	cellular component movement
GO:0007049	cell cycle
GO:0007155	cell adhesion
GO:0007186	G-protein coupled receptor signaling pathway
GO:0007264	small GTPase mediated signal transduction
GO:0015031	protein transport
GO:0016192	vesicle-mediated transport
GO:0030838	positive regulation of actin filament polymerization
GO:0030866	cortical actin cytoskeleton organization
GO:0031529	ruffle organization
GO:0033028	myeloid cell apoptotic process
GO:0034394	protein localization to cell surface
GO:0035020	regulation of Rac protein signal transduction
GO:0036010	protein localization to endosome
GO:0045087	innate immune response (InnateDB)
GO:0048261	negative regulation of receptor-mediated endocytosis
GO:0051301	cell division
GO:0051489	regulation of filopodium assembly
GO:0060998	regulation of dendritic spine development
GO:0090004	positive regulation of establishment of protein localization to plasma membrane
GO:0090162	establishment of epithelial cell polarity
GO:0097284	hepatocyte apoptotic process

Cellular Component

GO:0001726	ruffle
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005768	endosome
GO:0005769	early endosome
GO:0005794	Golgi apparatus
GO:0005886	plasma membrane
GO:0005938	cell cortex
GO:0016020	membrane
GO:0030139	endocytic vesicle
GO:0030496	midbody
GO:0031527	filopodium membrane
GO:0032154	cleavage furrow
GO:0055037	recycling endosome
GO:0055038	recycling endosome membrane
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR001019 Guanine nucleotide binding protein (G-protein), alpha subunit
IPR001806 Small GTPase superfamily
IPR003579 Small GTPase superfamily, Rab type
IPR005225 Small GTP-binding protein domain
IPR006687 Small GTPase superfamily, SAR1-type
IPR006689 Small GTPase superfamily, ARF/SAR type
IPR006762 Gtr1/RagA G protein
IPR013684 Mitochondrial Rho-like
IPR019009 Signal recognition particle receptor, beta subunit
IPR024156 Small GTPase superfamily, ARF type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00503
PF00071
PF00025
PF04670
PF08477
PF09439

PRINTS

PR00318
PR00449
PR00328

PIRSF

SMART

SM00275
SM00175
SM00178
SM00177

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt