InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RAD51

Summary

InnateDB Protein

IDBP-587422.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RAD51

Protein Name

RAD51 homolog (S. cerevisiae)

Synonyms

BRCC5; HRAD51; HsRad51; HsT16930; MRMV2; RAD51A; RECA;

Species

Homo sapiens

Ensembl Protein

ENSP00000433924

InnateDB Gene

IDBG-6509 (RAD51)

Protein Structure

UniProt Annotation

Function

Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2- scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. {ECO:0000269PubMed:12205100, ECO:0000269PubMed:12442171, ECO:0000269PubMed:18417535, ECO:0000269PubMed:20231364, ECO:0000269PubMed:20348101, ECO:0000269PubMed:20413593, ECO:0000269PubMed:23509288, ECO:0000269PubMed:23754376}.

Subcellular Localization

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.

Disease Associations

Breast cancer (BC) [MIM:114480]: A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. {ECO:0000269PubMed:10807537}. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.Mirror movements 2 (MRMV2) [MIM:614508]: A disorder characterized by contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities. {ECO:0000269PubMed:22305526}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Tissue Specificity

Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 117 experimentally validated interaction(s) in this database.
They are also associated with 9 interaction(s) predicted by orthology.

Experimentally validated
Total	117 [view]
Protein-Protein	112 [view]
Protein-DNA	5 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	9 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0003690	double-stranded DNA binding
GO:0003697	single-stranded DNA binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008022	protein C-terminus binding
GO:0008094	DNA-dependent ATPase activity
GO:0042802	identical protein binding
GO:0043142	single-stranded DNA-dependent ATPase activity
GO:0070182	DNA polymerase binding

Biological Process

GO:0000724	double-strand break repair via homologous recombination
GO:0000730	DNA recombinase assembly
GO:0006200	ATP catabolic process
GO:0006259	DNA metabolic process
GO:0006268	DNA unwinding involved in DNA replication
GO:0006281	DNA repair
GO:0006302	double-strand break repair
GO:0006310	DNA recombination
GO:0006312	mitotic recombination
GO:0006974	cellular response to DNA damage stimulus
GO:0007126	meiotic nuclear division
GO:0007131	reciprocal meiotic recombination
GO:0009432	SOS response
GO:0010569	regulation of double-strand break repair via homologous recombination
GO:0051106	positive regulation of DNA ligation
GO:0051260	protein homooligomerization
GO:0071479	cellular response to ionizing radiation
GO:0072757	cellular response to camptothecin

Cellular Component

GO:0000228	nuclear chromosome
GO:0000793	condensed chromosome
GO:0000794	condensed nuclear chromosome
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005759	mitochondrial matrix
GO:0005815	microtubule organizing center
GO:0016605	PML body
GO:0048471	perinuclear region of cytoplasm

Protein Structure and Domains

PDB ID

InterPro

IPR003593 AAA+ ATPase domain
IPR010995 DNA repair Rad51/transcription factor NusA, alpha-helical
IPR011941 DNA recombination/repair protein Rad51
IPR013632 DNA recombination and repair protein Rad51, C-terminal
IPR013765 DNA recombination and repair protein RecA
IPR016467 DNA recombination and repair protein, RecA-like
IPR020587 DNA recombination and repair protein RecA, monomer-monomer interface
IPR020588 DNA recombination and repair protein RecA-like, ATP-binding domain
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF08423
PF00154

PRINTS

PR00142

PIRSF

PIRSF005856

SMART

SM00382

TIGRFAMs

Post-translational Modifications

Modification

Cross-References