InnateDB Protein
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IDBP-590489.3
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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CHST4
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Protein Name
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carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
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Synonyms
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GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST;
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Species
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Homo sapiens
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Ensembl Protein
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ENSP00000441204
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InnateDB Gene
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IDBG-40958 (CHST4)
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Protein Structure
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Function |
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2- branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1- 3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location. {ECO:0000269PubMed:10330415}.
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Subcellular Localization |
Golgi apparatus membrane {ECO:0000269PubMed:12855678}; Single-pass type II membrane protein {ECO:0000269PubMed:12855678}.
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Disease Associations |
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Tissue Specificity |
Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma. {ECO:0000269PubMed:10330415, ECO:0000269PubMed:11310842, ECO:0000269PubMed:12107080}.
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
Experimentally validated |
Total |
1
[view]
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Protein-Protein |
1
[view]
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Protein-DNA |
0
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Molecular Function |
Accession |
GO Term |
GO:0001517
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N-acetylglucosamine 6-O-sulfotransferase activity
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GO:0008146
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sulfotransferase activity
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Biological Process |
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Cellular Component |
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PDB ID |
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InterPro |
IPR000863
Sulfotransferase domain
IPR016469
Carbohydrate sulfotransferase
IPR027417
P-loop containing nucleoside triphosphate hydrolase
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PFAM |
PF00685
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PRINTS |
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PIRSF |
PIRSF005883
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SMART |
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TIGRFAMs |
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Modification |
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SwissProt |
Q8NCG5
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PhosphoSite |
PhosphoSite-Q8NCG5
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TrEMBL |
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UniProt Splice Variant |
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Entrez Gene |
10164
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UniGene |
Hs.251383
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RefSeq |
NP_001159867
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HUGO |
HGNC:1972
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OMIM |
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CCDS |
CCDS10902
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HPRD |
07477
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IMGT |
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EMBL |
AF131235
AF149783
AF280088
AK074746
BC035282
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GenPept |
AAD33015
AAG48246
AAH35282
AAK48417
BAC11177
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