InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RUVBL2

Summary

InnateDB Protein

IDBP-61223.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RUVBL2

Protein Name

RuvB-like 2 (E. coli)

Synonyms

ECP51; INO80J; REPTIN; RVB2; TIH2; TIP48; TIP49B;

Species

Homo sapiens

Ensembl Protein

ENSP00000221413

InnateDB Gene

IDBG-61221 (RUVBL2)

Protein Structure

UniProt Annotation

Function

Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.

Subcellular Localization

Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.

Disease Associations

Tissue Specificity

Ubiquitously expressed. Highly expressed in testis and thymus.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 239 experimentally validated interaction(s) in this database.
They are also associated with 10 interaction(s) predicted by orthology.

Experimentally validated
Total	240 [view]
Protein-Protein	237 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	10 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003678	DNA helicase activity
GO:0005524	ATP binding
GO:0043141	ATP-dependent 5'-3' DNA helicase activity

Biological Process

GO:0006260	DNA replication
GO:0032508	DNA duplex unwinding

Cellular Component

Protein Structure and Domains

PDB ID

InterPro

IPR003959 ATPase, AAA-type, core
IPR007694 DNA helicase, DnaB-like, C-terminal
IPR010339 TIP49, C-terminal
IPR012340 Nucleic acid-binding, OB-fold
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00004
PF07724
PF13304
PF03796
PF06068

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

PhosphoSite

PhosphoSite-Q9Y230