InnateDB Protein
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IDBP-62941.7
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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DTNBP1
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Protein Name
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dystrobrevin binding protein 1
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Synonyms
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Species
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Homo sapiens
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Ensembl Protein
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ENSP00000344718
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InnateDB Gene
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IDBG-62935 (DTNBP1)
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Protein Structure
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Function |
Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway. {ECO:0000269PubMed:15345706, ECO:0000269PubMed:16837549, ECO:0000269PubMed:17182842, ECO:0000269PubMed:17989303, ECO:0000269PubMed:19094965, ECO:0000269PubMed:20180862, ECO:0000269PubMed:20921223}.
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Subcellular Localization |
Isoform 1: Cytoplasm {ECO:0000269PubMed:21390302}. Cytoplasmic vesicle membrane {ECO:0000269PubMed:21390302}; Peripheral membrane protein {ECO:0000269PubMed:21390302}; Cytoplasmic side {ECO:0000269PubMed:21390302}. Endosome membrane {ECO:0000269PubMed:21390302}; Peripheral membrane protein {ECO:0000269PubMed:21390302}; Cytoplasmic side {ECO:0000269PubMed:21390302}. Melanosome membrane {ECO:0000269PubMed:21390302}; Peripheral membrane protein {ECO:0000269PubMed:21390302}; Cytoplasmic side {ECO:0000269PubMed:21390302}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000269PubMed:21390302}. Endoplasmic reticulum {ECO:0000250}. Nucleus {ECO:0000269PubMed:21390302}. Note=Mainly cytoplasmic but shuttles between the cytoplasm and nucleus. Exported out of the nucleus via its NES in a XPO1-dependent manner. Nuclear localization is required for regulation of the expression of genes such as SYN1. Detected in neuron cell bodies, axons and dendrites. Mainly located to the postsynaptic density. Detected at tubulovesicular elements in the vicinity of the Golgi apparatus and of melanosomes. Occasionally detected at the membrane of pigmented melanosomes in cultured melanoma cells. The BLOC-1 complex associates with the BLOC-2 complex in early endosome-associated tubules.Isoform 2: Cytoplasm {ECO:0000269PubMed:21390302}. Cytoplasmic vesicle membrane {ECO:0000269PubMed:21390302}; Peripheral membrane protein {ECO:0000269PubMed:21390302}; Cytoplasmic side {ECO:0000269PubMed:21390302}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000269PubMed:21390302}; Peripheral membrane protein {ECO:0000269PubMed:21390302}; Cytoplasmic side {ECO:0000269PubMed:21390302}. Endosome membrane {ECO:0000269PubMed:21390302}; Peripheral membrane protein {ECO:0000269PubMed:21390302}; Cytoplasmic side {ECO:0000269PubMed:21390302}. Melanosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000269PubMed:21390302}. Endoplasmic reticulum {ECO:0000250}. Nucleus {ECO:0000269PubMed:21390302}. Note=Shuttles between the cytoplasm and nucleus. Exported out of the nucleus via its NES in a XPO1-dependent manner. Nuclear localization is required for regulation of the expression of genes such as SYN1. Mainly expressed in the dendritic spine. Predominantly a synaptic vesicle isoform but also highly expressed in the nucleus. The BLOC-1 complex associates with the BLOC-2 complex in early endosome-associated tubules. Associated with the AP-3 complex at presynaptic terminals.Isoform 3: Cytoplasm {ECO:0000269PubMed:21390302}. Cytoplasmic vesicle membrane {ECO:0000269PubMed:21390302}; Peripheral membrane protein {ECO:0000269PubMed:21390302}; Cytoplasmic side {ECO:0000269PubMed:21390302}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000269PubMed:21390302}; Peripheral membrane protein {ECO:0000269PubMed:21390302}; Cytoplasmic side {ECO:0000269PubMed:21390302}. Endosome membrane {ECO:0000269PubMed:21390302}; Peripheral membrane protein {ECO:0000269PubMed:21390302}; Cytoplasmic side {ECO:0000269PubMed:21390302}. Melanosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000269PubMed:21390302}. Endoplasmic reticulum {ECO:0000250}. Note=Exclusivley cytoplasmic. Predominantly found in the postsynaptic density (PSD). Little association with synaptic vesicles. The BLOC-1 complex associates with the BLOC-2 complex in early endosome-associated tubules. Associated with the AP-3 complex at presynaptic terminals.
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Disease Associations |
Hermansky-Pudlak syndrome 7 (HPS7) [MIM:614076]: A form of Hermansky-Pudlak syndrome, a genetically heterogeneous autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS. {ECO:0000269PubMed:12923531}. Note=The disease is caused by mutations affecting the gene represented in this entry.Note=Defects in DTNBP1 are associated with susceptibility to schizophrenia, a mental disorder characterized by a breakdown of thought processes and by poor emotional responsiveness. Genetic mutations lead to alterations in the glutamatergic transmisssion in the brain and modified Akt signaling (PubMed:15345706). Protein levels and expression are reduced in nerve terminals of the hippocampus and there is an increased release of glutamate in schizophrenic patients (PubMed:15124027). Levels of isoform 1 are reduced in the pSTG, but not in HF, by about 48% in 92% of schizophrenic patients. In the HF, there is an average of 33% reduction in synaptic expression of isoform 2 in 67% of cases, and of isoform 3, an average reduction of 35% in 80% of cases. In the dorsolateral prefrontal cortex (DLPFC), significant reductions in levels of isoform 3 are observed about 71% of schizophrenic patients showed an average reduction of this isoform of about 60% (PubMed:19617633). {ECO:0000269PubMed:15124027, ECO:0000269PubMed:15345706, ECO:0000269PubMed:19617633}.
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Tissue Specificity |
Detected in brain, in neurons and in neuropil. Isoform 1 is expressed in the cerebral cortex, and hippocampal frontal (HF). Specific expression in the posterior half of the superior temporal gyrus (pSTG). Higher expression of isoform 2 and 3 in the HF than in the pSTG while isoform 1 shows no difference in expression in these areas. In the HF, detected in dentate gyrus (DG) and in pyramidal cells of hippocampus CA2 and CA3 (at protein level). Expressed in all principal neuronal populations of the HF, namely pyramidal neurons in the subiculum and CA1-3, granule cells in the dense cell layer of the DG (DGg), and polymorph cells in the hilus of the DG (DGh). Maximal levels in CA2, CA3, and DGh. Isoform 2 not expressed in the cerebral cortex. {ECO:0000269PubMed:16980328}.
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 45 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.
Experimentally validated |
Total |
45
[view]
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Protein-Protein |
45
[view]
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Protein-DNA |
0
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Predicted by orthology |
Total |
3 [view]
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Molecular Function |
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Biological Process |
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Cellular Component |
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PDB ID |
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InterPro |
IPR001926
Tryptophan synthase beta subunit-like PLP-dependent enzyme
IPR007531
Dysbindin
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PFAM |
PF00291
PF04440
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PRINTS |
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PIRSF |
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SMART |
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TIGRFAMs |
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Modification |
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SwissProt |
Q96EV8
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PhosphoSite |
PhosphoSite-Q96EV8
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TrEMBL |
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UniProt Splice Variant |
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Entrez Gene |
84062
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UniGene |
Hs.571148
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RefSeq |
NP_898861
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HUGO |
HGNC:17328
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OMIM |
607145
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CCDS |
CCDS4535
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HPRD |
06190
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IMGT |
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EMBL |
AF061734
AF394226
AK054593
AK290718
AL021978
AL022343
AL136637
AY265460
BC011912
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GenPept |
AAG43145
AAH11912
AAL46636
AAP91870
BAB70770
BAF83407
CAB66572
CAI21976
CAI21977
CAI42339
CAI42340
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