InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TRIM28

Summary

InnateDB Protein

IDBP-73193.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TRIM28

Protein Name

tripartite motif containing 28

Synonyms

KAP1; PPP1R157; RNF96; TF1B; TIF1B;

Species

Homo sapiens

Ensembl Protein

ENSP00000253024

InnateDB Gene

IDBG-73191 (TRIM28)

Protein Structure

UniProt Annotation

Function

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. {ECO:0000269PubMed:10347202, ECO:0000269PubMed:11959841, ECO:0000269PubMed:15882967, ECO:0000269PubMed:16107876, ECO:0000269PubMed:16862143, ECO:0000269PubMed:17079232, ECO:0000269PubMed:17178852, ECO:0000269PubMed:17704056, ECO:0000269PubMed:17942393, ECO:0000269PubMed:18060868, ECO:0000269PubMed:18082607, ECO:0000269PubMed:20424263, ECO:0000269PubMed:20858735, ECO:0000269PubMed:20864041, ECO:0000269PubMed:21940674, ECO:0000269PubMed:23665872, ECO:0000269PubMed:8769649, ECO:0000269PubMed:9016654}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:10330177, ECO:0000269PubMed:17178852, ECO:0000269PubMed:20858735, ECO:0000269PubMed:23665872, ECO:0000269PubMed:9016654}. Note=Associated with centromeric heterochromatin during cell differentiation through CBX1. {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. {ECO:0000269PubMed:9016654}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 271 experimentally validated interaction(s) in this database.
They are also associated with 19 interaction(s) predicted by orthology.

Experimentally validated
Total	271 [view]
Protein-Protein	271 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	19 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0003713	transcription coactivator activity
GO:0003714	transcription corepressor activity
GO:0004672	protein kinase activity
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0031625	ubiquitin protein ligase binding
GO:0035851	Krueppel-associated box domain binding
GO:0043565	sequence-specific DNA binding
GO:0044822	poly(A) RNA binding
GO:0070087	chromo shadow domain binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0001837	epithelial to mesenchymal transition
GO:0006281	DNA repair
GO:0006367	transcription initiation from RNA polymerase II promoter
GO:0006468	protein phosphorylation
GO:0010467	gene expression
GO:0016567	protein ubiquitination
GO:0016925	protein sumoylation
GO:0042993	positive regulation of transcription factor import into nucleus
GO:0045087	innate immune response (InnateDB)
GO:0045739	positive regulation of DNA repair
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated
GO:0046777	protein autophosphorylation
GO:0051259	protein oligomerization
GO:0060028	convergent extension involved in axis elongation
GO:0060669	embryonic placenta morphogenesis
GO:1902187	negative regulation of viral release from host cell

Cellular Component

GO:0000785	chromatin
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005719	nuclear euchromatin
GO:0005720	nuclear heterochromatin

Protein Structure and Domains

PDB ID

InterPro

IPR000315 Zinc finger, B-box
IPR001487 Bromodomain
IPR001841 Zinc finger, RING-type
IPR001965 Zinc finger, PHD-type
IPR003649 B-box, C-terminal
IPR011011 Zinc finger, FYVE/PHD-type
IPR019787 Zinc finger, PHD-finger

PFAM

PF00643
PF00439
PF13639
PF14634
PF00628

PRINTS

PR00503

PIRSF

SMART

SM00336
SM00297
SM00184
SM00249
SM00502

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt