InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RNPS1

Summary

InnateDB Protein

IDBP-749528.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RNPS1

Protein Name

Synonyms

E5.1;

Species

Homo sapiens

Ensembl Protein

ENSP00000457723

InnateDB Gene

IDBG-10321 (RNPS1)

Protein Structure

UniProt Annotation

Function

Part of pre- and post-splicing multiprotein mRNP complexes. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Enhances the formation of the ATP-dependent A complex of the spliceosome. Involved in both constitutive splicing and, in association with SRP54 and TRA2B/SFRS10, in distinctive modulation of alternative splicing in a substrate-dependent manner. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Participates in mRNA 3'-end cleavage. Involved in UPF2- dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Also mediates increase of mRNA abundance and translational efficiency. Binds spliced mRNA 20-25 nt upstream of exon-exon junctions. {ECO:0000269PubMed:10449421, ECO:0000269PubMed:11546874, ECO:0000269PubMed:12665594, ECO:0000269PubMed:12944400, ECO:0000269PubMed:14729963, ECO:0000269PubMed:14752011, ECO:0000269PubMed:15684395, ECO:0000269PubMed:16209946, ECO:0000269PubMed:17586820, ECO:0000269PubMed:22203037}.

Subcellular Localization

Nucleus. Nucleus speckle. Cytoplasm. Note=Nucleocytoplasmic shuttling protein. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.

Disease Associations

Tissue Specificity

Ubiquitous. {ECO:0000269PubMed:8543184}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 145 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.

Experimentally validated
Total	145 [view]
Protein-Protein	142 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	3 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003730	mRNA 3'-UTR binding
GO:0005515	protein binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0000184	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0000381	regulation of alternative mRNA splicing, via spliceosome
GO:0000398	mRNA splicing, via spliceosome
GO:0006351	transcription, DNA-templated
GO:0006366	transcription from RNA polymerase II promoter
GO:0006369	termination of RNA polymerase II transcription
GO:0006406	mRNA export from nucleus
GO:0008380	RNA splicing
GO:0010467	gene expression
GO:0016070	RNA metabolic process
GO:0016071	mRNA metabolic process
GO:0031124	mRNA 3'-end processing
GO:0043065	positive regulation of apoptotic process
GO:0048025	negative regulation of mRNA splicing, via spliceosome

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0016607	nuclear speck
GO:0035145	exon-exon junction complex
GO:0061574	ASAP complex