InnateDB Protein
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IDBP-761296.3
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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RFFL
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Protein Name
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Synonyms
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Species
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Homo sapiens
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Ensembl Protein
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ENSP00000463035
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InnateDB Gene
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IDBG-41038 (RFFL)
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Protein Structure
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Function |
E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Also ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate apoptosis downstream of death domain receptors. Also negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SNF. May also play a role in endocytic recycling. {ECO:0000269PubMed:15069192, ECO:0000269PubMed:17121812, ECO:0000269PubMed:18382127, ECO:0000269PubMed:18450452, ECO:0000269PubMed:22609986}.
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Subcellular Localization |
Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Recycling endosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=The FYVE-type zinc finger may mediate phosphatidylinositol phosphate- binding and control subcellular localization.
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Disease Associations |
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Tissue Specificity |
Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. {ECO:0000269PubMed:15069192}.
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 18 experimentally validated interaction(s) in this database.
Experimentally validated |
Total |
18
[view]
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Protein-Protein |
18
[view]
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Protein-DNA |
0
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Molecular Function |
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Biological Process |
GO:0006511
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ubiquitin-dependent protein catabolic process
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GO:0010762
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regulation of fibroblast migration
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GO:0010804
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negative regulation of tumor necrosis factor-mediated signaling pathway
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GO:0032006
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regulation of TOR signaling
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GO:0043161
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proteasome-mediated ubiquitin-dependent protein catabolic process
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GO:0070936
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protein K48-linked ubiquitination
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GO:1901797
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negative regulation of signal transduction by p53 class mediator
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GO:1902042
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negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
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GO:2001271
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negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
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Cellular Component |
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PDB ID |
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InterPro |
IPR001841
Zinc finger, RING-type
IPR011011
Zinc finger, FYVE/PHD-type
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PFAM |
PF13639
PF14634
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PRINTS |
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PIRSF |
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SMART |
SM00184
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TIGRFAMs |
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Modification |
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SwissProt |
Q8WZ73
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PhosphoSite |
PhosphoSite-Q8WZ73
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TrEMBL |
J3QR17
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UniProt Splice Variant |
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Entrez Gene |
117584
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UniGene |
Hs.619546
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RefSeq |
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HUGO |
HGNC:24821
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OMIM |
609735
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CCDS |
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HPRD |
11489
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IMGT |
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EMBL |
AC004223
AF434816
AK093112
AY098935
BC015681
BC028424
CH471147
CR933651
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GenPept |
AAH15681
AAH28424
AAL30771
AAM29181
BAC04059
CAI45952
EAW80172
EAW80173
EAW80176
EAW80177
EAW80178
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