InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: XRCC6

Summary

InnateDB Protein

IDBP-9325.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

XRCC6

Protein Name

X-ray repair complementing defective repair in Chinese hamster cells 6

Synonyms

CTC75; CTCBF; G22P1; KU70; ML8; TLAA;

Species

Homo sapiens

Ensembl Protein

ENSP00000352257

InnateDB Gene

IDBG-9323 (XRCC6)

Protein Structure

UniProt Annotation

Function

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose- 5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. {ECO:0000269PubMed:12145306, ECO:0000269PubMed:20383123, ECO:0000269PubMed:20493174, ECO:0000269PubMed:2466842, ECO:0000269PubMed:7957065, ECO:0000269PubMed:8621488, ECO:0000269PubMed:9742108}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:22442688}. Chromosome {ECO:0000269PubMed:22442688}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 347 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	347 [view]
Protein-Protein	341 [view]
Protein-DNA	6 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0003690	double-stranded DNA binding
GO:0003691	double-stranded telomeric DNA binding
GO:0004003	ATP-dependent DNA helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008022	protein C-terminus binding
GO:0042162	telomeric DNA binding
GO:0044212	transcription regulatory region DNA binding
GO:0044822	poly(A) RNA binding
GO:0051575	5'-deoxyribose-5-phosphate lyase activity

Biological Process

GO:0000723	telomere maintenance
GO:0006266	DNA ligation
GO:0006281	DNA repair
GO:0006302	double-strand break repair
GO:0006303	double-strand break repair via nonhomologous end joining
GO:0006351	transcription, DNA-templated
GO:0006974	cellular response to DNA damage stimulus
GO:0007420	brain development
GO:0010212	response to ionizing radiation
GO:0016032	viral process
GO:0032481	positive regulation of type I interferon production
GO:0032508	DNA duplex unwinding
GO:0033151	V(D)J recombination
GO:0045087	innate immune response (InnateDB)
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0050769	positive regulation of neurogenesis
GO:0071475	cellular hyperosmotic salinity response
GO:0071481	cellular response to X-ray
GO:0075713	establishment of integrated proviral latency

Cellular Component

GO:0000783	nuclear telomere cap complex
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005667	transcription factor complex
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0016020	membrane
GO:0043234	protein complex
GO:0043564	Ku70:Ku80 complex
GO:0070419	nonhomologous end joining complex

Protein Structure and Domains

PDB ID

InterPro

IPR002035 von Willebrand factor, type A
IPR003034 SAP domain
IPR005160 Ku70/Ku80 C-terminal arm
IPR005161 Ku70/Ku80, N-terminal alpha/beta
IPR006164 Ku70/Ku80 beta-barrel domain
IPR006165 Ku70
IPR016194 SPOC like C-terminal domain

PFAM

PF00092
PF02037
PF03730
PF03731
PF02735

PRINTS

PIRSF

PIRSF003033

SMART

SM00327
SM00513
SM00559

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt