InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RPA2

Summary

InnateDB Protein

IDBP-95080.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RPA2

Protein Name

replication protein A2, 32kDa

Synonyms

REPA2; RP-A p32; RP-A p34; RPA32;

Species

Homo sapiens

Ensembl Protein

ENSP00000363021

InnateDB Gene

IDBG-95078 (RPA2)

Protein Structure

UniProt Annotation

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. {ECO:0000269PubMed:15205463, ECO:0000269PubMed:17765923, ECO:0000269PubMed:17959650, ECO:0000269PubMed:19116208, ECO:0000269PubMed:20154705, ECO:0000269PubMed:21504906, ECO:0000269PubMed:2406247, ECO:0000269PubMed:7697716, ECO:0000269PubMed:7700386, ECO:0000269PubMed:8702565, ECO:0000269PubMed:9430682, ECO:0000269PubMed:9765279}.

Subcellular Localization

Nucleus. Nucleus, PML body. Note=Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 483 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	483 [view]
Protein-Protein	478 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	2 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0003697	single-stranded DNA binding
GO:0005515	protein binding
GO:0019899	enzyme binding
GO:0019903	protein phosphatase binding
GO:0031625	ubiquitin protein ligase binding

Biological Process

GO:0000082	G1/S transition of mitotic cell cycle
GO:0000278	mitotic cell cycle
GO:0000718	nucleotide-excision repair, DNA damage removal
GO:0000722	telomere maintenance via recombination
GO:0000723	telomere maintenance
GO:0000724	double-strand break repair via homologous recombination
GO:0000730	DNA recombinase assembly
GO:0006260	DNA replication
GO:0006271	DNA strand elongation involved in DNA replication
GO:0006281	DNA repair
GO:0006283	transcription-coupled nucleotide-excision repair
GO:0006284	base-excision repair
GO:0006289	nucleotide-excision repair
GO:0006297	nucleotide-excision repair, DNA gap filling
GO:0006298	mismatch repair
GO:0006302	double-strand break repair
GO:0006310	DNA recombination
GO:0010569	regulation of double-strand break repair via homologous recombination
GO:0031571	mitotic G1 DNA damage checkpoint
GO:0032201	telomere maintenance via semi-conservative replication
GO:2000001	regulation of DNA damage checkpoint

Cellular Component

GO:0000781	chromosome, telomeric region
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005662	DNA replication factor A complex
GO:0016605	PML body
GO:0035861	site of double-strand break

Protein Structure and Domains

PDB ID

InterPro

IPR004365 OB-fold nucleic acid binding domain, AA-tRNA synthetase-type
IPR012340 Nucleic acid-binding, OB-fold
IPR014646 Replication factor A protein 2
IPR014892 Replication protein A, C-terminal

PFAM

PF01336
PF08784

PRINTS

PIRSF

PIRSF036949

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P15927