InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: MAGOH

Summary

InnateDB Protein

IDBP-98728.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

MAGOH

Protein Name

mago-nashi homolog, proliferation-associated (Drosophila)

Synonyms

MAGOH1; MAGOHA;

Species

Homo sapiens

Ensembl Protein

ENSP00000360525

InnateDB Gene

IDBG-98726 (MAGOH)

Protein Structure

UniProt Annotation

Function

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator WIBG/PYM leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. {ECO:0000269PubMed:12730685, ECO:0000269PubMed:16209946, ECO:0000269PubMed:22203037}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:19324961}. Nucleus speckle {ECO:0000269PubMed:19324961}. Cytoplasm {ECO:0000269PubMed:19324961}. Note=Detected in granule-like structures in the dendroplasm (By similarity). Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles. {ECO:0000250}.

Disease Associations

Tissue Specificity

Ubiquitous.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 202 experimentally validated interaction(s) in this database.

Experimentally validated
Total	202 [view]
Protein-Protein	198 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0000184	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0000381	regulation of alternative mRNA splicing, via spliceosome
GO:0000398	mRNA splicing, via spliceosome
GO:0006366	transcription from RNA polymerase II promoter
GO:0006369	termination of RNA polymerase II transcription
GO:0006406	mRNA export from nucleus
GO:0006417	regulation of translation
GO:0008380	RNA splicing
GO:0010467	gene expression
GO:0016070	RNA metabolic process
GO:0016071	mRNA metabolic process
GO:0031124	mRNA 3'-end processing

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005829	cytosol
GO:0016607	nuclear speck
GO:0035145	exon-exon junction complex
GO:0071013	catalytic step 2 spliceosome