InnateDB Protein
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IDBP-100399.6
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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RWDD3
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Protein Name
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RWD domain containing 3
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Synonyms
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RSUME;
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Species
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Homo sapiens
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Ensembl Protein
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ENSP00000359221
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InnateDB Gene
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IDBG-100397 (RWDD3)
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Protein Structure
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Function |
Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the: binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination. {ECO:0000269PubMed:17956732, ECO:0000269PubMed:22009797, ECO:0000269PubMed:23469069, ECO:0000269PubMed:23508108}.
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Subcellular Localization |
Nucleus {ECO:0000269PubMed:17956732}. Cytoplasm {ECO:0000269PubMed:17956732}. Note=Colocalizes with UBC9/UBE2I in nuclear spots.
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Disease Associations |
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Tissue Specificity |
Isoform 1 and isoform 2 are expressed in glioma tumors (at protein level). Expressed in a wide number of tissues with highest expression in cerebellum, pituitary, heart, kidney, liver, stomach, pancreas, prostate and spleen. Low levels in thalamus, spinal cord, esophagus, thymus, lung and peripheral blood leukocytes. A higher level expression seen in pituitary tumors as compared to the pituitary gland. {ECO:0000269PubMed:17956732, ECO:0000269PubMed:22009797, ECO:0000269PubMed:23469069}.
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 5 experimentally validated interaction(s) in this database.
Experimentally validated |
Total |
5
[view]
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Protein-Protein |
5
[view]
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Protein-DNA |
0
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Molecular Function |
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Biological Process |
GO:0032088
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negative regulation of NF-kappaB transcription factor activity
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GO:0033235
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positive regulation of protein sumoylation
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GO:1902073
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positive regulation of hypoxia-inducible factor-1alpha signaling pathway
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Cellular Component |
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PDB ID |
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InterPro |
IPR006575
RWD domain
IPR016135
Ubiquitin-conjugating enzyme/RWD-like
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PFAM |
PF05773
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PRINTS |
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PIRSF |
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SMART |
SM00591
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TIGRFAMs |
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Modification |
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SwissProt |
Q9Y3V2
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PhosphoSite |
PhosphoSite-Q9Y3V2
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TrEMBL |
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UniProt Splice Variant |
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Entrez Gene |
25950
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UniGene |
Hs.616913
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RefSeq |
NP_056300
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HUGO |
HGNC:21393
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OMIM |
615875
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CCDS |
CCDS41357
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HPRD |
11527
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IMGT |
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EMBL |
AC092802
AK292665
AK292699
AL050062
BC010936
BC125142
CR533493
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GenPept |
AAI25143
BAF85354
BAF85388
CAB43254
CAG38524
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