InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RWDD3

Summary

InnateDB Protein

IDBP-100401.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RWDD3

Protein Name

RWD domain containing 3

Synonyms

RSUME;

Species

Homo sapiens

Ensembl Protein

ENSP00000263893

InnateDB Gene

IDBG-100397 (RWDD3)

Protein Structure

UniProt Annotation

Function

Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the: binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination. {ECO:0000269PubMed:17956732, ECO:0000269PubMed:22009797, ECO:0000269PubMed:23469069, ECO:0000269PubMed:23508108}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:17956732}. Cytoplasm {ECO:0000269PubMed:17956732}. Note=Colocalizes with UBC9/UBE2I in nuclear spots.

Disease Associations

Tissue Specificity

Isoform 1 and isoform 2 are expressed in glioma tumors (at protein level). Expressed in a wide number of tissues with highest expression in cerebellum, pituitary, heart, kidney, liver, stomach, pancreas, prostate and spleen. Low levels in thalamus, spinal cord, esophagus, thymus, lung and peripheral blood leukocytes. A higher level expression seen in pituitary tumors as compared to the pituitary gland. {ECO:0000269PubMed:17956732, ECO:0000269PubMed:22009797, ECO:0000269PubMed:23469069}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 5 experimentally validated interaction(s) in this database.

Experimentally validated
Total	5 [view]
Protein-Protein	5 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding

Biological Process

GO:0032088	negative regulation of NF-kappaB transcription factor activity
GO:0033235	positive regulation of protein sumoylation
GO:1902073	positive regulation of hypoxia-inducible factor-1alpha signaling pathway