InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RAB13

Summary

InnateDB Protein

IDBP-102845.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RAB13

Protein Name

RAB13, member RAS oncogene family

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000357564

InnateDB Gene

IDBG-102843 (RAB13)

Protein Structure

UniProt Annotation

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin- induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis. {ECO:0000269PubMed:12058051, ECO:0000269PubMed:15096524, ECO:0000269PubMed:15528189, ECO:0000269PubMed:16525024, ECO:0000269PubMed:18779367, ECO:0000269PubMed:20008558}.

Subcellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Cell junction, tight junction. Golgi apparatus, trans-Golgi network membrane. Recycling endosome membrane. Cell projection, lamellipodium {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250}. Note=Tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions. Relocalizes to the leading edge of lamellipodia in migrating endothelial cells.

Disease Associations

Tissue Specificity

Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 19 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	19 [view]
Protein-Protein	19 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003924	GTPase activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0034236	protein kinase A catalytic subunit binding

Biological Process

GO:0006184	GTP catabolic process
GO:0006886	intracellular protein transport
GO:0006913	nucleocytoplasmic transport
GO:0007165	signal transduction
GO:0007264	small GTPase mediated signal transduction
GO:0010737	protein kinase A signaling
GO:0015031	protein transport
GO:0016197	endosomal transport
GO:0030866	cortical actin cytoskeleton organization
GO:0031175	neuron projection development
GO:0032456	endocytic recycling
GO:0032869	cellular response to insulin stimulus
GO:0035767	endothelial cell chemotaxis
GO:0044795	trans-Golgi network to recycling endosome transport
GO:0061024	membrane organization
GO:0070830	tight junction assembly
GO:0072659	protein localization to plasma membrane
GO:0090002	establishment of protein localization to plasma membrane
GO:0097368	establishment of Sertoli cell barrier
GO:1902463	protein localization to cell leading edge

Cellular Component

GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0005802	trans-Golgi network
GO:0005886	plasma membrane
GO:0005911	cell-cell junction
GO:0005923	tight junction
GO:0016020	membrane
GO:0016328	lateral plasma membrane
GO:0030027	lamellipodium
GO:0030139	endocytic vesicle
GO:0030658	transport vesicle membrane
GO:0030659	cytoplasmic vesicle membrane
GO:0031410	cytoplasmic vesicle
GO:0032593	insulin-responsive compartment
GO:0043005	neuron projection
GO:0055037	recycling endosome
GO:0055038	recycling endosome membrane
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR000795 Elongation factor, GTP-binding domain
IPR001806 Small GTPase superfamily
IPR002041 Ran GTPase
IPR003578 Small GTPase superfamily, Rho type
IPR003579 Small GTPase superfamily, Rab type
IPR005225 Small GTP-binding protein domain
IPR006689 Small GTPase superfamily, ARF/SAR type
IPR006762 Gtr1/RagA G protein
IPR013684 Mitochondrial Rho-like
IPR019009 Signal recognition particle receptor, beta subunit
IPR020849 Small GTPase superfamily, Ras type
IPR024156 Small GTPase superfamily, ARF type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00009
PF00071
PF00025
PF04670
PF08477
PF09439

PRINTS

PR00315
PR00449
PR00627
PR00328

PIRSF

SMART

SM00176
SM00174
SM00175
SM00173
SM00177

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt