InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: EPRS

Summary

InnateDB Protein

IDBP-106729.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

EPRS

Protein Name

glutamyl-prolyl-tRNA synthetase

Synonyms

EARS; GLUPRORS; PARS; QARS; QPRS;

Species

Homo sapiens

Ensembl Protein

ENSP00000355890

InnateDB Gene

IDBG-106727 (EPRS)

Protein Structure

UniProt Annotation

Function

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop- containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. {ECO:0000269PubMed:15479637, ECO:0000269PubMed:1756734, ECO:0000269PubMed:23071094}.

Subcellular Localization

Cytoplasm {ECO:0000305}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 95 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	95 [view]
Protein-Protein	94 [view]
Protein-DNA	0
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0004812	aminoacyl-tRNA ligase activity
GO:0004818	glutamate-tRNA ligase activity
GO:0004827	proline-tRNA ligase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016876	ligase activity, forming aminoacyl-tRNA and related compounds
GO:0035613	RNA stem-loop binding

Biological Process

GO:0006412	translation
GO:0006418	tRNA aminoacylation for protein translation
GO:0006424	glutamyl-tRNA aminoacylation
GO:0006433	prolyl-tRNA aminoacylation
GO:0006461	protein complex assembly
GO:0010467	gene expression
GO:0017148	negative regulation of translation
GO:0043039	tRNA aminoacylation
GO:0071346	cellular response to interferon-gamma

Cellular Component

GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0016020	membrane
GO:0030529	ribonucleoprotein complex
GO:0097452	GAIT complex

Protein Structure and Domains

PDB ID

InterPro

IPR000738 WHEP-TRS
IPR000924 Glutamyl/glutaminyl-tRNA synthetase
IPR002314 Aminoacyl-tRNA synthetase, class II (G/ H/ P/ S), conserved domain
IPR002316 Proline-tRNA ligase, class IIa
IPR004154 Anticodon-binding
IPR004499 Proline-tRNA ligase, class IIa, archaeal-type
IPR004526 Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolic
IPR006195 Aminoacyl-tRNA synthetase, class II
IPR009068 S15/NS1, RNA-binding
IPR010987 Glutathione S-transferase, C-terminal-like
IPR011035 Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain
IPR016061 Proline-tRNA ligase, class II, C-terminal
IPR017449 Prolyl-tRNA synthetase, class II
IPR020058 Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain
IPR020059 Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain

PFAM

PF00458
PF00587
PF03129
PF09180
PF00749
PF03950

PRINTS

PR00987
PR01046

PIRSF

SMART

SM00991
SM00946

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt