InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Ciita

Summary

InnateDB Protein

IDBP-132347.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Ciita

Protein Name

class II transactivator

Synonyms

C2ta; EG669998; Gm9475;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000023147

InnateDB Gene

IDBG-132343 (Ciita)

Protein Structure

UniProt Annotation

Function

Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription, the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: phosphorylates the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus. Nucleus, PML body {ECO:0000250}. Note=Recruited to PML body by PML. {ECO:0000250}.

Disease Associations

Tissue Specificity

Isoform 1 is expressed at very high levels in dendritic cells, at very low levels in spleen and thymus and is not detected in other tissues. Isoform 2 is detected at high levels in spleen and tonsil as well as in a number of B-lymphocyte cell lines, and at very low levels in dendritic cells. {ECO:0000269PubMed:9184229}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 26 experimentally validated interaction(s) in this database.
They are also associated with 47 interaction(s) predicted by orthology.

Experimentally validated
Total	26 [view]
Protein-Protein	10 [view]
Protein-DNA	16 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	47 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008022	protein C-terminus binding
GO:0032403	protein complex binding
GO:0033613	activating transcription factor binding
GO:0044212	transcription regulatory region DNA binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006351	transcription, DNA-templated
GO:0006954	inflammatory response
GO:0007568	aging
GO:0034341	response to interferon-gamma
GO:0045345	positive regulation of MHC class I biosynthetic process
GO:0045348	positive regulation of MHC class II biosynthetic process
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0046677	response to antibiotic
GO:0071257	cellular response to electrical stimulus
GO:0071346	cellular response to interferon-gamma
GO:0071360	cellular response to exogenous dsRNA

Cellular Component

GO:0009986	cell surface
GO:0016605	PML body

Protein Structure and Domains

PDB ID

MGI:108445

InterPro

IPR001611 Leucine-rich repeat
IPR003590 Leucine-rich repeat, ribonuclease inhibitor subtype
IPR007111 NACHT nucleoside triphosphatase
IPR008095 MHC class II transactivator
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00560
PF13504
PF13855

PRINTS

PR01719

PIRSF

SMART

SM00368

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt