InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Lats1

Summary

InnateDB Protein

IDBP-132502.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Lats1

Protein Name

large tumor suppressor

Synonyms

AW208599;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000041915

InnateDB Gene

IDBG-132500 (Lats1)

Protein Structure

UniProt Annotation

Function

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 4 experimentally validated interaction(s) in this database.
They are also associated with 44 interaction(s) predicted by orthology.

Experimentally validated
Total	4 [view]
Protein-Protein	4 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	44 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000287	magnesium ion binding
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0019901	protein kinase binding

Biological Process

GO:0000086	G2/M transition of mitotic cell cycle
GO:0000819	sister chromatid segregation
GO:0006468	protein phosphorylation
GO:0007067	mitotic nuclear division
GO:0009755	hormone-mediated signaling pathway
GO:0030216	keratinocyte differentiation
GO:0030833	regulation of actin filament polymerization
GO:0033138	positive regulation of peptidyl-serine phosphorylation
GO:0034613	cellular protein localization
GO:0035329	hippo signaling
GO:0045736	negative regulation of cyclin-dependent protein serine/threonine kinase activity
GO:0051220	cytoplasmic sequestering of protein
GO:0090090	negative regulation of canonical Wnt signaling pathway

Cellular Component

GO:0000922	spindle pole
GO:0005737	cytoplasm
GO:0005815	microtubule organizing center

Protein Structure and Domains

PDB ID

MGI:1333883

InterPro

IPR000449 Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminal
IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR009060 UBA-like
IPR011009 Protein kinase-like domain
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote
IPR017892 Protein kinase, C-terminal
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00627
PF00069
PF07714
PF00433

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00165
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt