InnateDB Protein
|
IDBP-133890.6
|
Last Modified
|
2014-10-13 [Report errors or provide feedback]
|
Gene Symbol
|
Podxl
|
Protein Name
|
podocalyxin-like
|
Synonyms
|
AW121214; Ly102; PC; PCLP-1; Pclp1;
|
Species
|
Mus musculus
|
Ensembl Protein
|
ENSMUSP00000026698
|
InnateDB Gene
|
IDBG-133888 (Podxl)
|
Protein Structure
|
|
Function |
Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. {ECO:0000269PubMed:11435469, ECO:0000269PubMed:17311105}.
|
Subcellular Localization |
Apical cell membrane {ECO:0000269PubMed:17311105}. Cell projection, microvillus {ECO:0000269PubMed:17311105}. Membrane raft {ECO:0000250}. Cell projection, lamellipodium {ECO:0000250}. Cell projection, filopodium {ECO:0000250}. Cell projection, ruffle {ECO:0000250}. Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}. Note=In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans- Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity). {ECO:0000250}.
|
Disease Associations |
|
Tissue Specificity |
Expressed in liver cells and hematopoietic cells (at protein level). Glomerular epithelium cell (podocyte). {ECO:0000269PubMed:11435469}.
|
Comments |
|
Number of Interactions
|
This gene and/or its encoded proteins are associated with 6 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.
Experimentally validated |
Total |
6
[view]
|
Protein-Protein |
6
[view]
|
Protein-DNA |
0
|
Protein-RNA |
0
|
DNA-DNA |
0
|
RNA-RNA |
0
|
DNA-RNA |
0
|
|
Predicted by orthology |
Total |
6 [view]
|
|
|
Molecular Function |
|
Biological Process |
|
Cellular Component |
|
PDB ID |
MGI:1351317
|
InterPro |
IPR013836
CD34/Podocalyxin
IPR017403
Podocalyxin-like protein 1
|
PFAM |
PF06365
|
PRINTS |
|
PIRSF |
PIRSF038143
|
SMART |
|
TIGRFAMs |
|
Modification |
|
SwissProt |
Q9R0M4
|
PhosphoSite |
PhosphoSite-Q9R0M4
|
TrEMBL |
Q791G4
|
UniProt Splice Variant |
|
Entrez Gene |
27205
|
UniGene |
Mm.89918
|
RefSeq |
NP_038751
|
MGI ID |
|
MGI Symbol |
Podxl
|
OMIM |
|
CCDS |
CCDS19983
|
HPRD |
|
IMGT |
|
EMBL |
AB028048
AF290205
AF290206
AF290207
AF290208
AF290209
BC052442
BC054530
CH466533
|
GenPept |
AAG02458
AAH52442
AAH54530
AAL27890
BAA86912
EDL13712
|
|
|