Mus musculus Protein: Ddx58
InnateDB Protein IDBP-135959.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol Ddx58
Protein Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Species Mus musculus
Ensembl Protein ENSMUSP00000042433
InnateDB Gene IDBG-135957 (Ddx58)
Protein Structure
UniProt Annotation
Function Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'- triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK- related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: newcastle disease virus (NDV) and Sendai virus (SeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and orthoreovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration. {ECO:0000269PubMed:16039576, ECO:0000269PubMed:16625202, ECO:0000269PubMed:17942531, ECO:0000269PubMed:19576794, ECO:0000269PubMed:19609254, ECO:0000269PubMed:19631370}.
Subcellular Localization Cytoplasm {ECO:0000250}. Cell projection, ruffle membrane {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cell junction, tight junction {ECO:0000250}. Note=Colocalized with TRIM25 at cytoplasmic perinuclear bodies. Associated with the actin cytoskeleton at membrane ruffles. {ECO:0000250}.
Disease Associations
Tissue Specificity
Number of Interactions This gene and/or its encoded proteins are associated with 33 experimentally validated interaction(s) in this database.
They are also associated with 52 interaction(s) predicted by orthology.
Experimentally validated
Total 33 [view]
Protein-Protein 20 [view]
Protein-DNA 2 [view]
Protein-RNA 11 [view]
Predicted by orthology
Total 52 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003690 double-stranded DNA binding
GO:0003725 double-stranded RNA binding
GO:0003727 single-stranded RNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016817 hydrolase activity, acting on acid anhydrides
GO:0042802 identical protein binding
Biological Process
GO:0002230 positive regulation of defense response to virus by host
GO:0008152 metabolic process
GO:0009597 detection of virus
GO:0009615 response to virus
GO:0030334 regulation of cell migration
GO:0032727 positive regulation of interferon-alpha production
GO:0032728 positive regulation of interferon-beta production
GO:0039529 RIG-I signaling pathway
GO:0042993 positive regulation of transcription factor import into nucleus
GO:0043330 response to exogenous dsRNA
GO:0045087 innate immune response (InnateDB)
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005923 tight junction
GO:0015629 actin cytoskeleton
GO:0032587 ruffle membrane
Protein Structure and Domains
PDB ID MGI:2442858
InterPro IPR001650 Helicase, C-terminal
IPR006935 Helicase/UvrB domain
IPR011029 Death-like domain
IPR011545 DNA/RNA helicase, DEAD/DEAH box type, N-terminal
IPR014001 Helicase, superfamily 1/2, ATP-binding domain
IPR021673 C-terminal domain of RIG-I
IPR027417 P-loop containing nucleoside triphosphate hydrolase
PFAM PF00271
Post-translational Modifications
SwissProt Q6Q899
PhosphoSite PhosphoSite-Q6Q899
UniProt Splice Variant
Entrez Gene 230073
UniGene Mm.86382
RefSeq NP_766277
MGI Symbol Ddx58
EMBL AK049305 AK078287 AK087261 AL831793 AY553221 CH466538
GenPept AAS59532 BAC33670 BAC37205 BAC39830 CAM27574 EDL05445