InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rasgrp2

Summary

InnateDB Protein

IDBP-136383.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rasgrp2

Protein Name

RAS, guanyl releasing protein 2

Synonyms

Caldaggef1; CDC25L;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000041135

InnateDB Gene

IDBG-136381 (Rasgrp2)

Protein Structure

UniProt Annotation

Function

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. {ECO:0000269PubMed:10777492, ECO:0000269PubMed:10913189, ECO:0000269PubMed:11278453, ECO:0000269PubMed:11432821, ECO:0000269PubMed:12239348, ECO:0000269PubMed:15334074, ECO:0000269PubMed:16357324, ECO:0000269PubMed:17492052, ECO:0000269PubMed:9789079}.

Subcellular Localization

Cytoplasm, cytosol {ECO:0000250}. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Cell junction, synapse, synaptosome {ECO:0000250}. Cell projection, ruffle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Note=Found both in the cytosol and associated with membranes. Enriched at juxtamembrane areas and membrane ruffles. Localizes to the cell bodies and axons of striatal neurons. {ECO:0000250}.

Disease Associations

Tissue Specificity

Detected in megakaryocytes, platelet and neutrophils but not in lymphocytes (at protein level). Isoform 1 and isoform 3 are detected in brain basal glanglia, heart, lung, spleen, liver and kidney interstitial cells. {ECO:0000269PubMed:10835426, ECO:0000269PubMed:11278453, ECO:0000269PubMed:15334074}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 5 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	5 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005085	guanyl-nucleotide exchange factor activity
GO:0005509	calcium ion binding

Biological Process

GO:0007264	small GTPase mediated signal transduction
GO:0035556	intracellular signal transduction
GO:0043087	regulation of GTPase activity
GO:0051056	regulation of small GTPase mediated signal transduction

Cellular Component

GO:0005622	intracellular
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0030054	cell junction
GO:0032587	ruffle membrane
GO:0043005	neuron projection
GO:0045202	synapse

Protein Structure and Domains

PDB ID

MGI:1333849

InterPro

IPR000651 Ras-like guanine nucleotide exchange factor, N-terminal
IPR001895 Guanine-nucleotide dissociation stimulator CDC25
IPR002048 EF-hand domain
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding
IPR023578 Ras guanine nucleotide exchange factor, domain

PFAM

PF00618
PF00617
PF00036
PF13202
PF13405
PF00130

PRINTS

PIRSF

SMART

SM00229
SM00147
SM00054
SM00109

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9QUG9