Mus musculus Protein: Top3b | |||||||||||||
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Summary | |||||||||||||
InnateDB Protein | IDBP-137528.6 | ||||||||||||
Last Modified | 2014-10-13 [Report errors or provide feedback] | ||||||||||||
Gene Symbol | Top3b | ||||||||||||
Protein Name | topoisomerase (DNA) III beta | ||||||||||||
Synonyms | |||||||||||||
Species | Mus musculus | ||||||||||||
Ensembl Protein | ENSMUSP00000023465 | ||||||||||||
InnateDB Gene | IDBG-137526 (Top3b) | ||||||||||||
Protein Structure | |||||||||||||
UniProt Annotation | |||||||||||||
Function | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity. {ECO:0000250}. | ||||||||||||
Subcellular Localization | |||||||||||||
Disease Associations | |||||||||||||
Tissue Specificity | Highly expressed in testis. | ||||||||||||
Comments | |||||||||||||
Interactions | |||||||||||||
Number of Interactions |
This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 11 interaction(s) predicted by orthology.
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Gene Ontology | |||||||||||||
Molecular Function |
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Biological Process |
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Cellular Component |
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Protein Structure and Domains | |||||||||||||
PDB ID | MGI:1333803 | ||||||||||||
InterPro |
IPR000380
DNA topoisomerase, type IA IPR003601 DNA topoisomerase, type IA, domain 2 IPR003602 DNA topoisomerase, type IA, DNA-binding IPR006171 Toprim domain IPR013497 DNA topoisomerase, type IA, central IPR023405 DNA topoisomerase, type IA, core domain |
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PFAM |
PF01751
PF13662 PF01131 |
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PRINTS |
PR00417
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PIRSF | |||||||||||||
SMART |
SM00436
SM00437 SM00493 |
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TIGRFAMs | |||||||||||||
Post-translational Modifications | |||||||||||||
Modification | |||||||||||||
Cross-References | |||||||||||||
SwissProt | Q9Z321 | ||||||||||||
PhosphoSite | PhosphoSite-Q9Z321 | ||||||||||||
TrEMBL | Q3UJF5 | ||||||||||||
UniProt Splice Variant | |||||||||||||
Entrez Gene | 21976 | ||||||||||||
UniGene | Mm.326089 | ||||||||||||
RefSeq | NP_035754 | ||||||||||||
MGI ID | |||||||||||||
MGI Symbol | Top3b | ||||||||||||
OMIM | |||||||||||||
CCDS | CCDS27990 | ||||||||||||
HPRD | |||||||||||||
IMGT | |||||||||||||
EMBL | AB013603 AC166832 AK144709 AK146475 BC031723 CH466521 | ||||||||||||
GenPept | AAH31723 BAA34227 BAE26026 BAE27200 EDK97432 | ||||||||||||