InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rgs14

Summary

InnateDB Protein

IDBP-155455.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rgs14

Protein Name

regulator of G-protein signaling 14

Synonyms

RPIP1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000068731

InnateDB Gene

IDBG-155453 (Rgs14)

Protein Structure

UniProt Annotation

Function

Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division; required for completion of the first mitotic division of the embryo. Involved in visual memory processing capacity; when overexpressed in the V2 secondary visual cortex area. Involved in hippocampal-based learning and memory; acts as a suppressor of synaptic plasticity in CA2 neurons. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance. {ECO:0000269PubMed:10926822, ECO:0000269PubMed:15112653, ECO:0000269PubMed:15525537, ECO:0000269PubMed:15917656, ECO:0000269PubMed:16246175, ECO:0000269PubMed:20837545}.

Subcellular Localization

Nucleus. Nucleus, PML body. Cytoplasm. Membrane. Cell membrane {ECO:0000250}. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, dendrite. Cell projection, dendritic spine. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Localizes with spindle poles during metaphase. Shuttles between the nucleus and cytoplasm in a CRM1- dependent manner. Recruited from the cytosol to the plasma membrane by the inactive GDP-bound forms of G(i) alpha subunits GNAI1 and GNAI3. Recruited from the cytosol to membranes by the active GTP-bound form of HRAS. Colocalizes with G(i) alpha subunit GNAI1 and RIC8A at the plasma membrane. Colocalizes with BRAF and RAF1 in both the cytoplasm and membranes (By similarity). Associates with the perinuclear sheaths of microtubules (MTs) surrounding the pronuclei, prior to segregating to the anastral mitotic apparatus and subsequently the barrel- shaped cytoplasmic bridge between the nascent nuclei of the emerging 2-cell embryo. Localizes to a perinuclear compartment near the microtubule- organizing center (MTOC). Expressed in the nucleus during interphase and segregates to the centrosomes and astral MTs during mitosis. Shuttles between the nucleus and cytoplasm in a CRM1- dependent manner. Relocalizes to the nucleus in PML nuclear bodies in respons to heat stress. Colocalizes with RIC8A in CA2 hippocampal neurons. {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in pyramidal neurons of the CA1, CA2 and fasciola cinerea (FC) subregions of the hippocampus and in the olfactory cortex (at protein level). Expressed in brain, spleen, heart, liver, lung, kidney, skin and thymus (at protein level). Expressed in granular layer of the cerebellum, forbrain, striatum, layer V of the cortex, olfactory cortex, tubercules, subthalamic and hippocampus, particularly in the CA2 region, to a lesser extent in the CA1 region and the external layer of the dentate gyrus. Expressed in neurons. {ECO:0000269PubMed:10926822, ECO:0000269PubMed:15525537, ECO:0000269PubMed:20837545, ECO:0000269PubMed:21158412}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 17 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	17 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001965	G-protein alpha-subunit binding
GO:0005057	receptor signaling protein activity
GO:0005092	GDP-dissociation inhibitor activity
GO:0005096	GTPase activator activity
GO:0005515	protein binding
GO:0008017	microtubule binding
GO:0019901	protein kinase binding
GO:0030159	receptor signaling complex scaffold activity
GO:0030695	GTPase regulator activity
GO:0032794	GTPase activating protein binding

Biological Process

GO:0006913	nucleocytoplasmic transport
GO:0006979	response to oxidative stress
GO:0007051	spindle organization
GO:0007059	chromosome segregation
GO:0007067	mitotic nuclear division
GO:0007165	signal transduction
GO:0007186	G-protein coupled receptor signaling pathway
GO:0007612	learning
GO:0007616	long-term memory
GO:0008277	regulation of G-protein coupled receptor protein signaling pathway
GO:0008542	visual learning
GO:0010070	zygote asymmetric cell division
GO:0031914	negative regulation of synaptic plasticity
GO:0035556	intracellular signal transduction
GO:0038032	termination of G-protein coupled receptor signaling pathway
GO:0043407	negative regulation of MAP kinase activity
GO:0043547	positive regulation of GTPase activity
GO:0043620	regulation of DNA-dependent transcription in response to stress
GO:0048008	platelet-derived growth factor receptor signaling pathway
GO:0050769	positive regulation of neurogenesis
GO:0051301	cell division
GO:0060291	long-term synaptic potentiation
GO:0070373	negative regulation of ERK1 and ERK2 cascade

Cellular Component

GO:0000922	spindle pole
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005813	centrosome
GO:0005819	spindle
GO:0005874	microtubule
GO:0005886	plasma membrane
GO:0014069	postsynaptic density
GO:0016604	nuclear body
GO:0016605	PML body
GO:0030054	cell junction
GO:0030425	dendrite
GO:0043197	dendritic spine
GO:0045111	intermediate filament cytoskeleton
GO:0045211	postsynaptic membrane

Protein Structure and Domains

PDB ID

MGI:1859709

InterPro

IPR000342 Regulator of G protein signalling domain
IPR003109 GoLoco motif
IPR003116 Raf-like Ras-binding
IPR016137 Regulator of G protein signalling superfamily
IPR029071 Ubiquitin-related domain

PFAM

PF00615
PF02188
PF02196

PRINTS

PR01301

PIRSF

SMART

SM00390
SM00455
SM00315

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt