InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Phf1

Summary

InnateDB Protein

IDBP-155715.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Phf1

Protein Name

PHD finger protein 1

Synonyms

AW557215; D17Ertd455e; mPcl1; PHF2; Tctex-3; Tctex3;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000073402

InnateDB Gene

IDBG-155713 (Phf1)

Protein Structure

UniProt Annotation

Function

Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18086877). According to other reports, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53. {ECO:0000269PubMed:18086877}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:18086877}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Note=Localizes specifically to the promoters of numerous target genes. Localizes to double-strand breaks (DSBs) sites following DNA damage. Colocalizes with NEK6 in the centrosome (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Testis-specific.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 5 experimentally validated interaction(s) in this database.
They are also associated with 19 interaction(s) predicted by orthology.

Experimentally validated
Total	5 [view]
Protein-Protein	5 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	19 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0035064	methylated histone binding

Biological Process

GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0006974	cellular response to DNA damage stimulus
GO:0016568	chromatin modification
GO:0061086	negative regulation of histone H3-K27 methylation
GO:0061087	positive regulation of histone H3-K27 methylation

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005815	microtubule organizing center
GO:0035098	ESC/E(Z) complex
GO:0035861	site of double-strand break

Protein Structure and Domains

PDB ID

MGI:98647

InterPro

IPR001965 Zinc finger, PHD-type
IPR002999 Tudor domain
IPR011011 Zinc finger, FYVE/PHD-type
IPR019787 Zinc finger, PHD-finger

PFAM

PF00567
PF00628

PRINTS

PIRSF

SMART

SM00249
SM00333

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9Z1B8

PhosphoSite

PhosphoSite-Q9Z1B8

TrEMBL

UniProt Splice Variant

Entrez Gene

21652

UniGene

RefSeq

NP_033369

MGI ID

MGI Symbol

Phf1

OMIM

CCDS

CCDS37518

HPRD

IMGT

EMBL

AB011550 U81490

GenPept

AAD00518 BAA25074