InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Pnpt1

Summary

InnateDB Protein

IDBP-156502.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Pnpt1

Protein Name

polyribonucleotide nucleotidyltransferase 1

Synonyms

1200003F12Rik; Old35; PNPase; Pnptl1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000020756

InnateDB Gene

IDBG-156498 (Pnpt1)

Protein Structure

UniProt Annotation

Function

RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double- stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. {ECO:0000269PubMed:20691904}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Mitochondrion {ECO:0000250}. Mitochondrion intermembrane space {ECO:0000269PubMed:16966381}; Peripheral membrane protein {ECO:0000269PubMed:16966381}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 17 interaction(s) predicted by orthology.

Predicted by orthology
Total	17 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000175	3'-5'-exoribonuclease activity
GO:0003723	RNA binding
GO:0004654	polyribonucleotide nucleotidyltransferase activity
GO:0005515	protein binding
GO:0008266	poly(U) RNA binding
GO:0034046	poly(G) binding
GO:0035198	miRNA binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0000957	mitochondrial RNA catabolic process
GO:0000958	mitochondrial mRNA catabolic process
GO:0000962	positive regulation of mitochondrial RNA catabolic process
GO:0000964	mitochondrial RNA 5'-end processing
GO:0000965	mitochondrial RNA 3'-end processing
GO:0006396	RNA processing
GO:0006401	RNA catabolic process
GO:0006402	mRNA catabolic process
GO:0034599	cellular response to oxidative stress
GO:0035458	cellular response to interferon-beta
GO:0035927	RNA import into mitochondrion
GO:0035928	rRNA import into mitochondrion
GO:0043457	regulation of cellular respiration
GO:0043631	RNA polyadenylation
GO:0045926	negative regulation of growth
GO:0051260	protein homooligomerization
GO:0061014	positive regulation of mRNA catabolic process
GO:0070207	protein homotrimerization
GO:0070584	mitochondrion morphogenesis
GO:0071042	nuclear polyadenylation-dependent mRNA catabolic process
GO:0071850	mitotic cell cycle arrest
GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic
GO:0097222	mitochondrial mRNA polyadenylation
GO:2000627	positive regulation of miRNA catabolic process
GO:2000772	regulation of cellular senescence

Cellular Component

GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005758	mitochondrial intermembrane space
GO:0005886	plasma membrane
GO:0045025	mitochondrial degradosome

Protein Structure and Domains

PDB ID

MGI:1918951

InterPro

IPR001247 Exoribonuclease, phosphorolytic domain 1
IPR003029 Ribosomal protein S1, RNA-binding domain
IPR004087 K Homology domain
IPR004088 K Homology domain, type 1
IPR012162 Polyribonucleotide nucleotidyltransferase
IPR012340 Nucleic acid-binding, OB-fold
IPR015847 Exoribonuclease, phosphorolytic domain 2
IPR015848 Polyribonucleotide nucleotidyltransferase, RNA-binding domain
IPR020568 Ribosomal protein S5 domain 2-type fold
IPR022967 RNA-binding domain, S1

PFAM

PF01138
PF00575
PF00013
PF13014
PF03725
PF03726

PRINTS

PIRSF

PIRSF005499

SMART

SM00322
SM00316

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt