InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: H2afx

Summary

InnateDB Protein

IDBP-156785.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

H2afx

Protein Name

H2A histone family, member X

Synonyms

AW228881; gammaH2ax; H2A.X; H2ax; Hist5-2ax;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000051432

InnateDB Gene

IDBG-156783 (H2afx)

Protein Structure

UniProt Annotation

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. {ECO:0000269PubMed:11740565, ECO:0000269PubMed:11934988, ECO:0000269PubMed:12034884, ECO:0000269PubMed:12447390, ECO:0000269PubMed:12660252, ECO:0000269PubMed:12689589, ECO:0000269PubMed:12792649, ECO:0000269PubMed:12914700, ECO:0000269PubMed:12914701, ECO:0000269PubMed:14530383, ECO:0000269PubMed:15574327, ECO:0000269PubMed:15580272, ECO:0000269PubMed:15589157, ECO:0000269PubMed:15610743, ECO:0000269PubMed:15632067}.

Subcellular Localization

Nucleus. Chromosome.

Disease Associations

Tissue Specificity

Most abundant in testis, thymus and spleen. {ECO:0000269PubMed:11242108}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 26 experimentally validated interaction(s) in this database.
They are also associated with 217 interaction(s) predicted by orthology.

Experimentally validated
Total	26 [view]
Protein-Protein	26 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	217 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0005515	protein binding
GO:0019899	enzyme binding
GO:0042393	histone binding
GO:0043565	sequence-specific DNA binding
GO:0046982	protein heterodimerization activity

Biological Process

GO:0000077	DNA damage checkpoint
GO:0000724	double-strand break repair via homologous recombination
GO:0006281	DNA repair
GO:0006334	nucleosome assembly
GO:0006974	cellular response to DNA damage stimulus
GO:0007126	meiotic nuclear division
GO:0007283	spermatogenesis

Cellular Component

GO:0000781	chromosome, telomeric region
GO:0000785	chromatin
GO:0000786	nucleosome
GO:0000790	nuclear chromatin
GO:0000794	condensed nuclear chromosome
GO:0001673	male germ cell nucleus
GO:0001741	XY body
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005657	replication fork
GO:0005694	chromosome
GO:0035861	site of double-strand break