InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Poli

Summary

InnateDB Protein

IDBP-157160.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Poli

Protein Name

polymerase (DNA directed), iota

Synonyms

Rad30b;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000039869

InnateDB Gene

IDBG-157158 (Poli)

Protein Structure

UniProt Annotation

Function

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000250}. Note=Accumulates at replication forks after DNA damage. {ECO:0000250}.

Disease Associations

Tissue Specificity

Detected in testis, and at very low levels in spleen, lung and brain. Detected in round spermatids, but not in prophase spermatocytes. {ECO:0000269PubMed:10458907}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 9 experimentally validated interaction(s) in this database.
They are also associated with 10 interaction(s) predicted by orthology.

Experimentally validated
Total	9 [view]
Protein-Protein	9 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	10 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003684	damaged DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0046872	metal ion binding

Biological Process

GO:0006261	DNA-dependent DNA replication
GO:0006281	DNA repair

Cellular Component

GO:0005634

nucleus

Protein Structure and Domains

PDB ID

MGI:1347081

InterPro

IPR001126 DNA-repair protein, UmuC-like
IPR017961 DNA polymerase, Y-family, little finger domain
IPR017963 DNA-repair protein, UmuC-like, N-terminal
IPR024728 DNA polymerase type-Y, HhH motif

PFAM

PF00817
PF11799
PF11798

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q6R3M4

PhosphoSite