InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RPA1

Summary

InnateDB Protein

IDBP-15787.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RPA1

Protein Name

replication protein A1, 70kDa

Synonyms

HSSB; MST075; REPA1; RF-A; RP-A; RPA70;

Species

Homo sapiens

Ensembl Protein

ENSP00000254719

InnateDB Gene

IDBG-15783 (RPA1)

Protein Structure

UniProt Annotation

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single- stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S- phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). {ECO:0000269PubMed:12791985, ECO:0000269PubMed:17765923, ECO:0000269PubMed:17959650, ECO:0000269PubMed:19116208, ECO:0000269PubMed:19996105, ECO:0000269PubMed:7697716, ECO:0000269PubMed:7700386, ECO:0000269PubMed:9430682, ECO:0000269PubMed:9765279}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:17959650}. Nucleus, PML body {ECO:0000269PubMed:17959650}. Note=Enriched in PML bodies in cells displaying alternative lengthening of their telomeres. {ECO:0000269PubMed:17959650}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 495 experimentally validated interaction(s) in this database.
They are also associated with 11 interaction(s) predicted by orthology.

Experimentally validated
Total	495 [view]
Protein-Protein	494 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	11 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003684	damaged DNA binding
GO:0003697	single-stranded DNA binding
GO:0005515	protein binding
GO:0046872	metal ion binding

Biological Process

GO:0000082	G1/S transition of mitotic cell cycle
GO:0000278	mitotic cell cycle
GO:0000718	nucleotide-excision repair, DNA damage removal
GO:0000722	telomere maintenance via recombination
GO:0000723	telomere maintenance
GO:0000724	double-strand break repair via homologous recombination
GO:0000730	DNA recombinase assembly
GO:0001701	in utero embryonic development
GO:0006260	DNA replication
GO:0006261	DNA-dependent DNA replication
GO:0006271	DNA strand elongation involved in DNA replication
GO:0006281	DNA repair
GO:0006283	transcription-coupled nucleotide-excision repair
GO:0006284	base-excision repair
GO:0006289	nucleotide-excision repair
GO:0006297	nucleotide-excision repair, DNA gap filling
GO:0006298	mismatch repair
GO:0006302	double-strand break repair
GO:0006310	DNA recombination
GO:0007126	meiotic nuclear division
GO:0008284	positive regulation of cell proliferation
GO:0030097	hemopoiesis
GO:0032201	telomere maintenance via semi-conservative replication
GO:0048873	homeostasis of number of cells within a tissue
GO:0051276	chromosome organization

Cellular Component

GO:0000793	condensed chromosome
GO:0000794	condensed nuclear chromosome
GO:0000800	lateral element
GO:0001673	male germ cell nucleus
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005662	DNA replication factor A complex
GO:0005737	cytoplasm
GO:0015629	actin cytoskeleton
GO:0016605	PML body

Protein Structure and Domains

PDB ID

InterPro

IPR004365 OB-fold nucleic acid binding domain, AA-tRNA synthetase-type
IPR004591 Replication factor A protein 1
IPR007199 Replication factor-A protein 1, N-terminal
IPR012340 Nucleic acid-binding, OB-fold
IPR013955 Replication factor A, C-terminal

PFAM

PF01336
PF04057
PF08646

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P27694

PhosphoSite